WEEK 32: 05 AUGUST 2024 RUN – JGK Lab AfriQA

Scheme Description and Code	Samples			Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
Scheme Description and Code	Type	Identification (as per the labels)	Storage	Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
Antibiogram – mastitis pathogens JGKPT02	2 x FD cultures 2 x Amies Swabs (BKP samples)	ATB01/M – Dairy cow ATB02/M – Dairy cow	2-8^oC	Freeze-dried samples (Primary samples): Allow cultures and sDH₂O to adjust to room temperature. Remove seals and bungs from the vials and add 300 µl sDH₂O to each vial. Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per your lab’s SOP). Using a sterile loop streak to facilitate discrete colonies Amies Swabs (Backup samples): To be used only as a backup if necessary.	These samples are not intended to be stored. Test the samples immediately upon receipt and reconstitution	Culturing: Micro Susceptibility: Disc diffusion (Kirby-Bauer) / Qualitative	R.E.F 02 (supplied)	Zone Ø in millimeters. Report results as: Sensitive (S), Intermediate (I), Resistance (R)	Culture: 1 rep Susceptibility: 3 replicates (supplied discs)
Antibiogram – mastitis pathogens JGKPT02	Antimicrobial discs (x 21)	Florfenicol 30 µg (x 7)/ Oxoid; Lot # 3753954; Exp. 12/04/2024 Penicillin/Novobiocin 40 (x 7)/ Oxoid; Lot # 3711351; Exp. 08/2024 Cephalothin 30 µg (x 7)/ Oxoid; Lot # 3591986; Exp. 03/12/2024	2-8^oC	Commercial discs: Ready to use			R.E.F 02 (supplied)
Bacteriology – Dog & Cat JGKPT13	3 x FD cultures 3 x Amies Swabs (Backup samples)	BAC01/DC – BAC03/DC	2-8^oC	Freeze-dried samples: Allow cultures and sDH₂O to adjust to room temperature. Remove seals and bungs from the vials and add 300 µl sDH₂O to each vial. Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per your lab’s SOP). Using a sterile loop streak to facilitate discrete colonies Amies Swabs (Backup samples): To be used only as a backup if necessary.	These samples are not intended to be stored. Test the samples immediately upon receipt and reconstitution	Culture & ID	R.E.F 11-16	Full bacterial identification	1 rep
Bacteriology – Horse JGKPT14	3 x FD cultures 3 x Amies Swabs (Backup samples)	BAC01/H – BAC03/H	2-8^oC			Culture & ID	R.E.F 11-16	Full bacterial identification	1 rep
Antimicrobial residues – meat JGKPT23-25	4 x Fresh samples (chicken liver)	RES-01-F – RES-04-F	2-8^oC	Fresh samples: Ready to process	Test immediately upon receipt	Rapid test Microbiology Chromatography	R.E.F 23-25 (supplied)	Presence/Absence of antimicrobials The ID of the antibiotic	1 rep
Antimicrobial residues – milk JGKPT20-22	4 x bovine bulk milk	RES-01-M – RES-04-M	2-8^oC	N/A Ready to process	Test immediately upon receipt	Rapid test Microbiology Chromatography	R.E.F 20-22 (supplied)	Presence/Absence of antimicrobials Name of the antibiotic	1 rep
Food hygiene – Detection JGKPT25-30	4 x Amies swabs	FH01 – FH04	2-8^oC	N/A Ready to process	Test immediately upon receipt	Microbiology (conventional & PCR)	R.E.F 25-30 (supplied)	ID of bacterial isolate or presence/absence of the target bacteria	1 rep
Food hygiene – Enumeration	4 x FD samples	FH01/Counts – FH04/Counts	2-8^oC	Allow samples to adjust to room temperature. Add 5 ml preheated (37^oC) sterile DH₂O to each jar. Mix content thoroughly on a vortex until there are no clumps	These samples are not intended to be stored. Test the samples immediately upon receipt and reconstitution	Microbiology (conventional & PCR)	R.E.F 25-30 (supplied)	Counts	TBC: 2 rep Coliforms: 2 rep E. coli: 2 rep
Mastitis pathogens – milk JGKPT34	1 x FD culture 1 x cow’s milk	MAST01 – MAST02	2-8^oC	Freeze-dried culture: Allow the culture and sDH₂O to adjust to room temperature. Remove the seal and bung from the vial and add 300 µl sDH₂O to the vial. Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per your lab’s SOP). Using a sterile loop streak to facilitate discrete colonies Milk: Ready to process	These samples are not intended to be stored. Test the samples immediately upon receipt and reconstitution	Culture & ID	R.E.F 33-34 (supplied)	Target bacteria	1 rep
Milk hygiene: TBC, Coliforms, and E. coli counts JGKPT17-19	4 x FD milk (bovine)	MH01 – MH04	2-8^oC	Add 5 ml sterile DH₂O to each vial Notes: Do not use cold water. Samples must adjust to room temperature for ≥ 30 min before adding sD.H₂O. D.H₂O must be at 37^oC in a water bath for ≥30 min before use. Mix the content thoroughly on a vortex until there are no clumps.	These samples are not intended to be stored. Test the samples immediately upon receipt and reconstitution	3M Petri Films Conventional technique	R.E.F 17-19	Counts	TBC: 2 rep Coliforms: 2 rep E. coli: 2 rep
Mycotoxins: Aflatoxins & Fumonisins JGKPT31 – 32	2 x maize meal	MYC01 – MYC02	2-8^oC	N/A Ready to process	Test immediately upon receipt/ reconstitution	Chromatography	R.E.F 31-32	Detection and quantification of Aflatoxins (in ppb) and Fumonisins (in ppm)	1 rep
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory. Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company. Specific environmental conditions required for the participant to conduct tests None Results recording Only the supplied Excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F. Submission of PT results to JGK All results must be submitted on or before Wednesday the 28^th of August 2024. Any results received after this date will not be included in the analysis. Enquiries For any inquiry, please use the contact details provided above to contact JGK Return of the proficiency test items None