PT Instructions

WEEK 20: 15 MAY 2023 RUN

PURPOSE

To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1

INSTRUCTIONS

Scheme Description and Code Samples Samples  reconstitution Testing timeframe Approved test methods Sheet to be used for submission of results to JGK Unit of measurement (Results) Number of test replicates per sample
Type Identification

(as per the labels)

 Storage
Antibiogram – mastitis pathogens

JGKPT02

 Nutrient agar plates and swabs (BKP) ATB01M – ATB02M

ATB01M: NA plate + swab

ATB02M: swab only

 2-8oC N/A

Ready to process

 Culturing of bacteria should be done Immediately upon receipt of the samples Culturing: Micro

Susceptibility: Kirby-Bauer / Disc diffusion (Qualitative)

 R.E.F 02

(supplied)

 Zone Ø in millimeters.

Report results as:

Sensitive (S), Intermediate (I), Resistance (R)

 Culture: 1 rep

Susceptibility:

3 replicates (supplied discs)
21 x Antibiotic discs 7 x Cephalexin 30 µg, Lot # QJ04a (Abtek)

7 x Tetracycline 30 µg, Lot # 3214586; exp. 14.01.24 (Oxoid)

7 x Penicillin G 10 IU, Lot # 3164322; exp. 21.10.23 (Oxoid)

 Commercial discs:

Ready to use
Antimicrobial residues – meat

JGKPT23-25

 4 x Fresh samples

(chicken liver)

 RES-01-F – RES-04-F 2-8oC Fresh samples: Ready to process Test immediately upon receipt Rapid test

Microbiology

Chromatography

 R.E.F 23-25

(supplied)

 Presence/Absence of antimicrobials

 

ID of the antibiotic

 1 rep
Antimicrobial residues – milk

JGKPT20-22

 4 x bovine milk RES-01-M – RES-04-M 2-8oC N/A

Ready to process

 Test immediately upon receipt Rapid test

Microbiology

Chromatography

 R.E.F 20-22

(supplied)

 Presence/Absence of antimicrobials

 

Name of the antibiotic

 1 rep
Food hygiene – meat (Detection)

JGKPT25-30

 4 x Amies swabs FH01 – FH04 2-8oC N/A

Ready to process

 Test immediately upon receipt Microbiology (conventional & PCR) R.E.F 25-30

(supplied)

 Presence/Absence of target bacteria FD: 1 rep

Swab: 1 rep
Food hygiene – meat (Enumeration) 4 x FD samples FH01/Counts – FH04/Counts 2-8oC Add 20 ml preheated (37oC) sterile DH2O

to each jar

 Test immediately upon receipt/

reconstitution

 Counts TBC: 2 rep

Coliforms: 2 rep

E. coli: 2 rep
Mastitis pathogens – milk

JGKPT34

 2 x Cows’ milk MAST01 – MAST02 2-8oC N/A

Ready to process

 Test immediately upon receipt Culture & ID R.E.F 33-34

(supplied)

 Target bacteria 1 rep
Milk hygiene: TBC, Coliforms and E. coli counts

JGKPT17-19

 4 x FD milk (bovine) MH01 – MH04 2-8oC Add 6 ml sterile DH2O

to each vial

Notes:

N.B: Do not use cold water. Samples must adjust to room To for ≥ 30 min before adding D.H2O.

D.H2O must be at 37oC in a water bath for ≥30 min before use

 Test immediately upon reconstitution 3M Petri Films

Conventional technique

 R.E.F 17-19 Counts TBC: 2 rep

Coliforms: 2 rep

E. coli: 2 rep
Aflatoxins & Fumonisins

JGKPT31-32

 3 x maize meal MYC01 – MYC03 2-8oC N/A

Ready to process

 Test immediately upon receipt Chromatography R.E.F 31-32 Detection & quantification of Aflatoxins (ppb) and Fumonisins (ppm) 1 rep.
BVD & Fasciola

JGKPT39-40

 5 x FD sera (bovine) BVD/FAS01 – BVD/FAS05 2-8oC Add sterile DH2O to the vials as follows:

Sample 1: 500 µL

Sample 2: 250 µL

Sample 3: 350 µL

Sample 4: 300 µL

Sample 5: 400 µL

 Test immediately upon reconstitution ELISA R.E.F 39-45 Elisa ODs 2 rep.
Avian influenza - Abs

JGKPT41

 6 x FD sera (chicken) AI01 – AI06 2-8oC The volume of sample is written on the vials and differs for some samples depending on whether the lab conducts HI/Elisa only or both.

Sample 1: 700 & 400 µL

Sample 2: 500 µL

Sample 3: 700 & 350 µL

Sample 4: 500 µL

Sample 5: 500 µL

Sample 6: 500 µL

 Test immediately upon reconstitution ELISA

HI

 R.E.F 39-45 ODs / Titres 2 rep
Inf. bronchitis virus – Abs

  JGKPT42

 6 x FD sera (chicken) IBV01 – IBV06 2-8oC The volume is not the same for all the samples: Add sD.H2O to the vials as follows:

Sample 1: 200 µL

Sample 2: 250 µL

Sample 3: 200 µL

Sample 4: 250 µL

Sample 5: 250 µL

Sample 6: 200 µL

 Test immediately upon receipt ELISA

HI

 R.E.F 39-45 ODs / Titres 2 rep
Newcastle disease – Abs JGKPT43 6 x FD sera (poultry) NDV01 – NDV06 2-8oC The volume is not the same for all the samples: Add sD.H2O to the vials as follows:

Sample 1: 250 µL

Sample 2: 400 µL

Sample 3: 250 µL

Sample 4: 250 µL

Sample 5: 400 µL

Sample 6: 250 µL

 Test immediately upon reconstitution ELISA

HI

 R.E.F 39-45 ODs / Titres 2 rep
Salmonella enteritidis – Abs

JGKPT44

 6 x FD sera (poultry) SE01 – SE06 2-8oC The volume is not the same for all the samples: Add sD.H2O to the vials as follows:

Sample 1: 250 µL

Sample 2: 200 µL

Sample 3: 200 µL

Sample 4: 200 µL

Sample 5: 200 µL

Sample 6: 250 µL

 Test immediately upon receipt ELISA

RPAT

 R.E.F 39-45 Positive / Negative 1 rep
Mycoplasma gallisepticum - Abs

JGKPT45

 6 x FD sera (poultry) MG01 – MG06 2-8oC The volume is not the same for all the samples: Add sD.H2O to the vials as follows:

Sample 1: 250 µL

Sample 2: 200 µL

Sample 3: 250 µL

Sample 4: 200 µL

Sample 5: 250 µL

Sample 6: 200 µL

 Test immediately upon receipt ELISA

RPAT

 R.E.F 39-45 Positive / Negative 1 rep
Treatment of samples

No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.

 

Handling and safety requirements

Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.

 

Specific environmental conditions required for the participant to conduct tests

None

 

Results recording

Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.

 

Submission of PT results to JGK

All results must be submitted on or before the 7th of June 2023. Any results received after this date will not be included in the analysis.

 

Enquiries

For any inquiry, please use the contact details provided above to contact JGK

 

Return of the proficiency test items

None

FD: Freeze-Dried