reference

(as per the labels)

Antimicrobial susceptibility

(Clin. Pathogens)

2 x Amies swabs: backup samples

1 x NA Plate with 2 grown bacteria: QC plate. Incubation can be extended if necessary

Notes:

1. Culturing must be done from the FD cultures (see reconstitution)

2. The swabs can be used as BKP samples if there is a problem.

3. The NA plate serves only as QC for the growth of the 2 FD culture

ATB02/C – Dog  isolate

Remove seals and bungs from the vials and add 400 µl sDH₂O to each vial.

Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per lab’s SOP). Using a sterile loop streak to facilitate discrete colonies.

Proceed with Antibiogram as per Lab’s SOP

Preferably, test the samples immediately upon receipt and upon reconstitution

(Kirby Bauer)

(available on www.jgklab.co.za)

Report results as:

Sensitive (S), Intermediate (I), Resistance (R)

Susceptibility:

Supplied discs: 3

(as per result sheet)

(x 18)

Cefpodoxime 10 µg (x 6)/ Oxoid; Lot # 3199565.

Sulphamethoxazole/Trimethoprim 25 µg (x6)/ Oxoid; Lot # 3552294.

Brucella: RBT

(Samples for labs doing RBT only)

Allow sera and sDH₂O to adjust to room temperature.

Remove seals and bungs, and add sDH₂O as follows:

Sample/vial 1: 300 µl

Sample/vial 2: 1 ml

Sample/vial 3: 200 µl

Sample/vial 4: 500 µl

Sample/vial 5: 500 µl

Sample/vial 6: 200 µl

Do not add cold water.

There must be no clumps in the serum when tested

(available on www.jgklab.co.za)

Or

Negative

Brucella: RBT/CFT

(Samples for labs doing both RBT and CFT)

Allow sera and sDH₂O to adjust to room temperature.

Remove seals and bungs, and add sDH₂O as follows:

Sample/vial 1: 500 µl

Sample/vial 2: 500 µl

Sample/vial 3: 500 µl

Sample/vial 4: 500 µl

Sample/vial 5: 500 µl

Sample/vial 6: 1 ml

Do not add cold water.

There must be no clumps in the serum when tested

(available on www.jgklab.co.za)

Positive (low, medium, high)

Or

Negative

Option 1: Endpoint reaction (e.g. 1/4 ++++, 1/128+)

Option 2: Titer (e.g. 24, 480)

NB: the corresponding serum dilution (e.g. 1/4) must always be indicated or ticked on the result sheet

2

Brucella: MRT

(not preserved)

Ready to process

(available on www.jgklab.co.za)

Or

Negative

Brucella: Culture & Identification

Allow cultures and sDH₂O to adjust to room temperature.

Remove seals and bungs, and add sDH₂O as follows:

Sample/vial 1: 300 µl

Sample/vial 2: 400 µl

Sample/vial 3: 400 µl

Sample/vial 4: 400 µl

Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per lab’s SOP). Using a sterile loop streak to facilitate discrete colonies

Preferably, test the samples immediately upon receipt and upon reconstitution

and

Molecular  technique

(available on www.jgklab.co.za)

Strain:

Field or vaccine

Brucella Slides

BA Slide 02: x 3

BA Slide 03: x 3

Dust-free

(available on www.jgklab.co.za)

Bacteriology: Listeria species (Clinical Micro)

3 x Amies swabs: Backup samples

Notes:

1. Culturing must be done from the FD cultures (see reconstitution)

2. The swabs are backup samples to be used only if there is a problem with primary cultures

Allow cultures and sDH₂O to adjust to room temperature.

Remove seals and bungs, and add sDH₂O as follows:

Sample/vial 1: 400 µl

Sample/vial 2: 400 µl

Sample/vial 3: 400 µl

Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per lab’s SOP). Using a sterile loop streak to facilitate discrete colonies

Preferably, test the samples immediately upon receipt and upon reconstitution

and

Molecular  technique

(available on www.jgklab.co.za)

Listeria:

Identify to Genus Level, Listeria, and to Species Level, e.g. monocytogenes)

Bacteriology: Salmonella in Poultry

(Clinical Micro)

3 x Amies swabs: Backup samples

Notes:

1. Culturing must be done from the FD cultures (see reconstitution)

2. The swabs are backup samples to be used only if there is a problem with primary cultures

Allow cultures and sDH₂O to adjust to room temperature.

Remove seals and bungs, and add sDH₂O as follows:

Sample/vial 1: 400 µl

Sample/vial 2: 300 µl

Sample/vial 3: 400 µl

Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per lab’s SOP). Using a sterile loop streak to facilitate discrete colonies

Preferably, test the samples immediately upon receipt and upon reconstitution

and

Molecular  technique

(available on www.jgklab.co.za)

Salmonella:

Identify to Genus Level, Salmonella, and to Serotype Level, e.g. Enteritidis)

Bacteriology: Salmonella in Mammals

(Clinical Micro)

3 x Amies swabs: Backup samples

Notes:

1. Culturing must be done from the FD cultures (see reconstitution)

2. The swabs are backup samples to be used only if there is a problem with primary cultures

Allow cultures and sDH₂O to adjust to room temperature.

Remove seals and bungs, and add sDH₂O as follows:

Sample/vial 1: 400 µl

Sample/vial 2: 300 µl

Sample/vial 3: 400 µl

Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per lab’s SOP). Using a sterile loop streak to facilitate discrete colonies

Preferably, test the samples immediately upon receipt and upon reconstitution

and

Molecular  technique

(available on www.jgklab.co.za)

Salmonella:

Identify to Genus Level, Salmonella, and to Serotype Level, e.g. Typhimurium)

Trichomonas and Campylobacter ID by Culture

Ready to process

(available on www.jgklab.co.za)

Trichomonas foetus/ Campylobacter fetus

Trichomonas and Campylobacter ID by PCR

No special treatment should be given to the PT samples. They [PT samples] should be handled in the same manner as all the other samples routinely tested in the laboratory, including TAT

Handling and safety requirements

Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.

Specific environmental conditions required for the participant to conduct tests

JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+

Results recording

Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The R.E.F grouped into WK8,19,31,43 and WK9,20,32,44 are available online (www.jgklab.co.za) and can be downloaded under “PUBLICATIONS”  The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.

Submission of PT results to JGK

All results must be submitted on Wednesday, 13^th of March 2024. Any results received after this date will not be included in the analysis.

 Enquiries

For any inquiry, please use the contact details provided above to contact JGK

 Return of the proficiency test items

None