PT Instructions
25 October 2021 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme Description and Code | Samples | Samples reconstitution | Testing timeframe | Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| Antibiogram – mastitis pathogens
JGKPT02 |
2 x Broths | ATB01M – ATB02M | 2-8oC | Ready to process | Culturing of bacteria should be done Immediately upon receipt of samples | Culturing: micro
Susceptibility: Kirby-Bauer / Disc diffusion (Qualitative) |
R.E.F 02
(supplied) |
Zone Ø in millimeters
(Results: S, I, R) |
Culture: 1 rep
Susceptibility: 3 replicates (supplied discs) |
| 18 x Antibiotic Discs | 6 x Tetracycline 30 µg
6 x Erythromycin 15 µg 6 x Florfenicol 30 µg |
Commercial discs:
Ready to process |
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| Antimicrobial residues – meat
JGKPT23-25 |
4 x Pieces of meat | RES-01-F – RES-04-F | 2-8oC | Ready to process | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 23-25
(supplied) |
Presence/Absence of antimicrobials
ID of the antibiotic |
1 rep |
| Antimicrobial residues – milk
JGKPT20-22 |
4 x Liquid milk (bovine) | RES-01-M – RES-04-M | 2-8oC | Ready to process | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 20-22
(supplied) |
Presence/Absence of antimicrobials
ID of the antibiotic |
1 rep |
| Food hygiene – meat (Detection)
JGKPT25-30 |
4 x Broths | FH01 – FH04 | 2-8oC | Ready to process | Test immediately upon receipt | Microbiology (conventional & PCR) | R.E.F 25-30
(supplied) |
Presence/Absence of target bacteria | 1 rep |
| Mastitis pathogens – milk
JGKPT34 |
2 x Milk (bovine) | MAST01 – MAST02 | 2-8oC | Ready to process | Test immediately upon receipt | Culture & ID | R.E.F 33-34
(supplied) |
Target bacteria | 1 rep |
| Milk hygiene: TBC, Coliforms and E. coli counts
JGKPT17-19 |
4 x FD milk | MH01 – MH04 | 2-8oC | Add 10 ml sterile DH2O to each vial Vortex /mix thoroughly before testing
NB: sample 1 (MH01): inoculate Petri films with 1:10 dilution |
Let the samples adjust to room temperature for 30 min before testing | 3M Petri Films
Conventional technique |
R.E.F 17-19 | Counts | 2 rep |
| Aflatoxins & Fumonisins
JGKPT31-32 |
3 x Maize meal
(100 g) |
MYC01 – MYC03 | 2-8oC | Ready to process | Test immediately upon receipt | Chromatography | R.E.F 31-32 | Presence & quantification of Aflatoxins (ppb) and Fumonisins (ppm) | 1 rep. |
| Somatic cells counts – milk
JGKPT33 |
6 x Preserved milk (bovine) | SCC01 – SCC06 | 2-8oC | Mix thoroughly before testing | Test within 24 hours of receipt | Somatic cells counting device | R.E.F 33-34
(supplied) |
Counts | 3 rep |
| Dog & cat Disease Scenarios JGKPT13 | 3 x Broths | BAC01/DC – BAC03/DC | 2-8oC | Ready to process | Test immediately upon receipt | Conventional microbiology and
Molecular technique |
R.E.F 11-16
(available on www.jgklab.co.za) |
Full ID of all the bacteria isolated in each sample | 1 |
| Horse Disease Scenarios JGKPT14 | 3 x Broths | BAC01/H – BAC03/H | 2-8oC | Ready to process | 1 | ||||
| BVD & Fasciola
JGKPT39-40 |
5 x Liquid bovine sera | BVD/FASC01 – BVD/FASC05 | 2-8oC | Ready to process | Test immediately upon receipt | ELISA | R.E.F 39-45 | Elisa ODs | 2 rep. |
| Avian influenza - Abs
JGKPT41 |
6 x FD poultry sera | AI01 – AI06 | 2-8oC | Add 500 µL sterile DH2O to each vial | Test immediately upon reconstitution | ELISA / HI | R.E.F 39-45 | ODs / Titres | 2 rep |
| Inf. bronchitis virus – Abs
JGKPT42 |
6 x FD poultry sera | IBV01 – IBV06 | 2-8oC | Add 400 µL sterile DH2O to each vial | Test immediately upon reconstitution | ELISA / HI | R.E.F 39-45 | ODs / Titres | 2 rep |
| Newcastle disease – Abs JGKPT43 | 4 x FD
2 x Liquid Poultry sera |
NDV01, 02, 03, 05: FD
NDV04, 06: Liquid |
2-8oC | Add 400 µL sterile DH2O to vials 1, 2, 3, 5
NDV04 & NDV06: Ready to process |
Test immediately upon receipt and reconstitution of the FD samples | ELISA / HI | R.E.F 39-45 | ODs / Titres | 2 rep |
| Salmonella enteritidis – Abs
JGKPT44 |
6 x FD poultry sera | SE01 – SE06 | 2-8oC | Add 400 µL sterile DH2O to each vial | Test immediately upon reconstitution | ELISA / RPAT | R.E.F 39-45 | Positive / Negative | 1 rep |
| Mycoplasma gallisepticum - Abs
JGKPT45 |
6 x FD poultry sera | MG01 – MG06 | 2-8oC | Add 400 µL sterile DH2O to each vial | Test immediately upon reconstitution | ELISA / RPAT | R.E.F 39-45 | Positive / Negative | 1 rep |
| Treatment of samples
No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests None
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 10th of November 2021. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None |
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FD: Freeze-Dried