PT Instructions
23 February 2021 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme Description and Code | Samples | Samples reconstitution | Testing timeframe | Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| Antibiogram – mastitis pathogens
JGKPT02 |
2 x FD cultures | ATB01M – ATB02M | 2-8oC | FD Cultures: add 1 ml sterile DH2O to ATB01 and 0.5 ml to ATB02
Discs: commercial, ready to use |
Culturing of bacteria should be done Immediately upon reconstitution
NB: important to note that some FD take longer to grow or may require to go on a broth first, then agar |
Culturing: micro
Susceptibility: Kirby-Bauer / Disc diffusion |
R.E.F 02
(supplied) |
Zone Ø in millimeters
(Results: S, I, R) |
Culture: 1 rep
Susceptibility: 3 replicates (supplied discs) |
| 18 x antibiotic discs | 6 x Gentamycin 10 µg
6 x Amikacin 30 µg 6 x Tetracycline 30 µg |
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| Antimicrobial residues – meat
JGKPT23-25 |
4 x pieces of meat | RES01-F – RES04-F | 2-8oC | Ready to process | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 23-25
(supplied) |
Presence/Absence of antimicrobials
ID of the antibiotic |
1 rep |
| Antimicrobial residues – milk
JGKPT20-22 |
4 x milk
(liquid) |
RES01-M – RES04-M | 2-8oC | Ready to process | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 20-22
(supplied) |
Presence/Absence of antimicrobials
ID of the antibiotic |
1 rep |
| Food hygiene – meat (Detection)
JGKPT25-30 |
4 x FD cultures (3 FD for 1 lab) | FH01 – FH04 | 2-8oC | Add 0.5 ml sterile DH2O to each vial | Test immediately upon reconstitution
NB: important to note that some FD take longer to grow or may require to go on a broth first, then agar |
Microbiology (conventional & automated) | R.E.F 25-30
(supplied) |
Presence/Absence of target bacteria | 1 rep |
| Mastitis pathogens – milk
JGKPT34 |
2 x FD Cultures | MAST01 – MAST02 | 2-8oC | Add 1 ml sterile DH2O to each vial | Test immediately upon reconstitution
NB: important to note that some FD take longer to grow or may require to go on a broth first, then agar |
Culture & ID | R.E.F 33-34
(supplied) |
Target bacteria | 1 rep |
| Milk hygiene: TBC, Coliforms and E. coli count
JGKPT17-19 |
4 x FD milk
2 x Milk for reconstitution |
MH01 – MH04 | 2-8oC | Allow the reconstitution milk to adjust to room Temp. for 5 min
Add 10 ml reconstitution milk to each vial/sample Allow the milk to dissolve for 5 min. Vortex /mix thoroughly before testing N.B: sample 3 (MH03): inoculate the PF with 1:10 inoculum No dilution for samples 1,2,4 |
Test within 4 hours of receipt | 3M Petri Films | R.E.F 17-19 | Counts | 2 rep |
| Somatic cells counts – milk
JGKPT33 |
6 x preserved milk
(20 ml) |
SCC01 – SCC06 | 2-8oC | Mix thoroughly before testing | Test within 48 hours of receipt | Somatic cells counting device | R.E.F 33-34
(supplied) |
Counts | 3 rep |
| Treatment of samples
No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests None
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 11th of March 2021. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
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FD: Freeze-Dried