PT Instructions
22 October 2019 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
Deadline for Submission of results: 13 November 2019
Results received after this date will not be included in the analysis.
| Scheme Description and Code | Samples | Samples reconstitution | Testing timeframe | Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| Antibiogram – mastitis pathogens
JGKPT02 |
2 x FD cultures | ATB01M-ATB02M | 2-8oC | FD Cultures: add 1 ml sterile D.H2O to each vial
Discs: ready for use |
Culturing of bacteria should be done Immediately upon reconstitution | Culturing: micro
Susceptibility: Kirby-Bauer / Disc diffusion |
R.E.F 02
(supplied) |
Zone Ø in millimeters
(Results: S, I, R) |
Culture: 1 rep
Susceptibility: 3 rep |
| 18 x antibiotic discs | 6 x Tetracycline 30 µg
6 x Cephalexin 30 µg 6 x Amikacin 30 µg |
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| Antimicrobial residues – meat
JGKPT24-26 |
2 x muscles
2 x livers (chicken) |
RES01-F – RES04-F | 2-8oC | Ready to process | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 24-26
(supplied) |
Presence/Absence of antimicrobials | 1 rep |
| Antimicrobial residues – milk
JGKPT21-23 |
4 x milk
(liquid) |
RES01-M – RES04-M | 2-8oC | Ready to process | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 21-23
(supplied) |
Presence/Absence of antimicrobials | 1 rep |
| BVD and Fasciola
JGKPT40-41 |
5 x serum (liquid) | BVD/FASC01 – BVD/FASC05 | 2-8oC | Ready to process | Test immediately upon receipt | ELISA | R.E.F 40-46
(supplied) |
ODs
Positive/Negative |
ODs: 2 rep
Pos/Neg: 1 rep |
| Poultry serology
JGKPT42-46 |
6 x serum each (liquid) | AI01 – AI06 | 2-8oC
|
Ready to process | Test immediately upon receipt | ELISA, HA/HI | R.E.F 40.46
(supplied) |
ODs, S/P, S/N | ODs: 2 rep
S/P / S/N: 2 rep Titres: 2 rep Pos/Neg: 1 rep |
| IBV01 – IBV06 | ELISA | ODs | |||||||
| NDV01 – NDV06 | ELISA, HA/HI | ODs, Titres | |||||||
| MG01 – MG06 | RPAT, ELISA | Agglutination, ODs | |||||||
| SE01 – SE06 | RPAT | Agglutination | |||||||
| Food hygiene – meat
JGKPT27-31 |
Detection
4 x meat |
FH01 – FH04 | 2-8oC | Ready to process | Test immediately upon receipt | Microbiology (conventional & automated) | R.E.F 27-31
(supplied) |
Presence/Absence of target bacteria | 1 rep |
| Enumeration
4 x FD samples |
TVC/COL/EC01 – TVC/COL/EC04 | 2-8oC | Add 10 ml sterile D.H2O to each vial | Test immediately upon reconstitution | Microbiology (conventional & automated) | R.E.F 27-31
(supplied) |
Colony forming units of target analytes | 2 rep | |
| Mastitis pathogens – milk
JGKPT35 |
2 x milk
(liquid) |
MAST01 – MAST02 | 2-8oC | Ready to process | Test immediately upon receipt | Culture & ID | R.E.F 34-35
(supplied) |
Target bacteria | 1 rep |
| Milk hygiene: TBC, Coliforms and E. coli count
JGKPT18-20 |
4 x FD milk | MH01 – MH04 | 2-8oC | Inoculate the PF using 1:10 diluted milk as follows:
Step 1: Reconstitute each sample with 10 ml sterile D.H2O. Mix thoroughly. No clumps, please!!
Step 2: Draw 1 ml from the reconstituted milk and add it to 9 ml sterile D.H2O in a separate sterile vessel Mix thoroughly. No clumps, please!!
|
Test immediately after making the 1:10 dilutions | 3M Petri Films | R.E.F 18-20 | Colony forming units | 2 rep |
| Mycotoxins – Aflatoxins and Fumonisins
JGKPT32-33 |
3 x maize flowers | MYC01 – MYC03 | 2-8oC | Ready to process | Test immediately upon receipt | Chromatography | R.E.F 32-33 | ppm | 2 rep |
| Somatic cells counts – milk
JGKPT34 |
6 x preserved milk
(20 ml) |
SCC01 – SCC06 | 2-8oC | Mix thoroughly before testing | Test immediately upon receipt | Somatic cells counting device | R.E.F 34-35
(supplied) |
Counts | 3 rep |
| Treatment of samples
No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests None
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 13th of November 2019. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
|
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FD: Freeze-Dried