PT Instructions
20 May 2019 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
Deadline for Submission of results: 19 June 2019
Results received after this date will not be included in the analysis.
| Scheme Description and Code | Samples | Samples reconstitution | Testing time frame | Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| Antibiogram – mastitis pathogens
JGKPT02 |
2 x FD cultures | ATB01M-ATB02M | 2-8oC | FD Cultures: add 1 ml sterile D.H2O to each vial
Discs: ready for use |
Culturing of bacteria should be done Immediately upon reconstitution | Culturing: micro
Susceptibility: Kirby-Bauer / Disc diffusion |
R.E.F 02
(supplied) |
Zone Ø in millimeters
(Results: S, I, R) |
Culture: 1 rep
Susceptibility: 3 rep |
| 18 x antibiotic discs | 6 x Ciprofloxacin 5 µg,
6 x Peni/Novo 40, 6 x Vancomycin 30 µg |
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| Antimicrobial residues – meat
JGKPT24-26 |
4 x chicken livers | RES01-F – RES04-F | 2-8oC | Ready to process | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 24-26
(supplied) |
Presence/Absence of antimicrobials | 1 rep |
| Antimicrobial residues – milk
JGKPT21-23 |
4 x milk
(liquid) |
RES01-M – RES04-M | 2-8oC | Ready to process | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 21-23
(supplied) |
Presence/Absence of antimicrobials | 1 rep |
| BVD and Fasciola
JGKPT40-41 |
5 x serum (liquid) | BVD & FASC01- BVD & FASC05 | 2-8oC | Ready to process | Test immediately upon receipt | ELISA | R.E.F 40-46
(supplied) |
ODs
Positive/Negative |
ODs: 2 rep
Pos/Neg: 1 rep |
| Poultry serology
JGKPT42-46 |
6 x serum each (liquid) | AI01-AI06: test AI only
NDV01-NDV06: test NDV only AI&NVD01-AI&NDV06: test both AI and NDV IBV&MG01- IBV&MG06: test both IBV and MG IBV/MG/SE01- IBV/MG/SE06: test IBV, MG and SE |
2-8oC
|
Ready to process | Test immediately upon receipt | ELISA
HA/HI RPAT |
R.E.F 40.46
(supplied) |
ODs
S/P Ratios Titres Positive/Negative |
ODs: 2 rep
S/P Ratios: 2 rep Titres: 2 rep Pos/Neg: 1 rep |
| Avian influenza and Newcastle disease
(PCR) JGKPT48, 50 |
3 x inactivated virus suspensions
(1.2 ml) |
AI-PCR01-AI-PCR03
ND-PCR01-ND-PCR03 |
2-8oC
Or -20oC (if not tested immediately |
Ready to process | Test immediately upon receipt | PCR | R.E.F 48,50
(supplied) |
Positive/Negative matrix & target nucleic acid | 1 rep |
| Food hygiene – meat (Detection)
JGKPT27-31 |
5 x pieces of meat | FH01 – FH05 | 2-8oC | Ready to process | Test immediately upon receipt | Microbiology (conventional & automated) | R.E.F 27-31
(supplied) |
Presence/Absence of target bacteria | 1 rep |
| Mastitis pathogens – milk
JGKPT35 |
2 x milk
(liquid) |
MAST01 – MAST02 | 2-8oC | Ready to process | Test immediately upon reconstitution | Culture & ID | R.E.F 34-35
(supplied) |
Target bacteria | 1 rep |
| Milk hygiene: TBC, Coliforms and E. coli count
JGKPT18-20 |
4 x milk
(liquid, 20 ml)
And
4 Inoculums (i.e. cultures to be used to inoculate the milk) |
Milk:
MH01 – MH04
Inoculums: Inoculum 01 – Inoculum 04 |
2-8oC | Milk:
Ready to use Inoculums: 1 Reconstitute each inoculum with 20 ml (twenty) sterile D.H2O using the supplied sterile 20 ml vials 2 Inoculation: add 50 µl (fifty) of each reconstituted inoculum to the corresponding milk (i.e. 50 µl of inoculum 1 to 20 ml of milk 1, 50 µl of inoculum 2 to 20 ml of milk 2, etc. Note: 1 The inoculum must be exactly 20 ml 2 Vortex the inoculated milk before inoculating the PF |
Test immediately after reconstitution of the inoculums | 3M Petri Films | R.E.F 18-20 | Counts | 2 rep
You can report 1 replicate if you want to save the petri films |
| Mycotoxins – Aflatoxins and Fumonisins
JGKPT32-33 |
3 x maize flowers | MYC01-MYC03 | 2-8oC | Ready to process | Test immediately upon receipt | Chromatography | R.E.F 32-33 | ppm | 2 rep |
| Somatic cells counts – milk
JGKPT34 |
6 x preserved milk
(20 ml) |
SCC01 – SCC06 | 2-8oC | Mix thoroughly before testing | Test immediately upon receipt | Somatic cells counting device | R.E.F 34-35
(supplied) |
Counts | 3 rep |
| Treatment of samples
No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests None
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 19th of June 2019. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
|
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FD: Freeze-Dried