PT Instructions
05 August 2019 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
Deadline for Submission of results: 28 August 2019
Results received after this date will not be included in the analysis.
| Scheme
reference |
Samples | Samples reconstitution | Testing timeframe | Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| JGKPT01
Antimicrobial susceptibility (clin. pathogens) |
Animal sp.: (1) Dog and (2) Cattle
2 FD cultures |
ATB01/C
ATB02/C |
2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution | Disc diffusion technique | R.E.F 1.2
(supplied) |
Zone Ø in millimeters.
Results: Sensitive (S), Intermediate (I), Resistance (R) |
Culture ID: 1
Susceptibility: Supplied discs: 3 (as per result sheet) |
| Antimicrobial discs (x18) | Florfenicol 30 µg (x6)
Doxycycline 30 µg (x6) Norfloxacin 10 µg (x6) |
Discs are ready to use (Commercial discs) | |||||||
| JGKPT03
Brucella: RBT (for RBT only) |
6 FD cattle sera
+ Positive control and RBT Antigen |
RBT01 – RBT06 | 2-8oC | Add 0.5 ml sterile DH2O to the vial | Test within 2 days of reconstitution | RBT
Please test using the supplied Antigen and Positive control |
R.E.F 3.5
(supplied) |
RBT:
Positive/Negative Using the supplied Antigen and Positive Control
CFT: CFTIU/ml
|
1 |
| JGKPT04
Brucella: CFT (for RBT & CFT) |
6 FD cattle sera
+ Positive control and RBT Antigen |
RBT/CFT01 – RBT/CFT06 | 2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution | CFT
(DAFF approved SOP for SA Labs) Please test RBT using the supplied Antigen and Positive control |
R.E.F 3.5
(supplied) |
2 | |
| JGKPT05
Brucella: MRT |
4 cow’s milk | CAM01 – CAM04 | 2-8oC | NA
Ready to process |
Preferably immediately but no later than 5 days from date of dispatch | MRT | R.E.F 3.5
(supplied) |
Positive/Negative | 1 |
| JGKPT06
Brucella: Culture & Identification |
3 Freeze dried cultures | CAC01 – CAC03 | 2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution | Conventional microbiology and
Molecular technique |
R.E.F 06
(supplied) |
Presence/Absence: Brucella abortus
Strain: Field or vaccine |
1 |
| JGKPT07
Brucella Slides |
3 unstained smears | Slide 1 - Slide 3 (x 3 each) | Room To
Dust-free |
Proceed with staining technique as per Lab SOP | When and as convenient, but before submission deadline | Stamp’s staining technique | R.E.F 06
(supplied) |
Positive/Negative for Brucella | 1 |
| JGKPT10
Salmonella Poultry |
3 Freeze dried cultures | SAP01 – SAP03 | 2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution | Conventional microbiology and
Molecular technique |
R.E.F 10.1
(supplied) |
Positive/Negative
ID of the isolate ID of the salmonella |
1 |
| JGKPT11
Salmonella Mammals |
3 Freeze dried cultures | SAM01 – SAM03 | 2-8oC | Add 1 ml sterile DH2O to the vial | Positive/Negative
ID of the salmonella |
1 | |||
| JGKPT13
Chickens diseases |
3 Freeze dried cultures | BA01/CH – BA03/CH | 2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution | Conventional microbiology, API and auto systems | R.E.F 12.7
(supplied) |
Full ID of the bacteria isolated, incl. the target bacteria | 1 |
| JGKPT14
Dog/cat diseases |
3 Freeze dried cultures | BA01/DC – BA03/DC | 2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution | Conventional microbiology, API and auto systems | R.E.F 12.7
(supplied) |
Full ID of the bacteria isolated, incl. the target bacteria | 1 |
| JGKPT16
Pig diseases |
3 Freeze dried cultures | BA01/P – BA03/P | 2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution | Conventional microbiology, API and auto systems | R.E.F 12.7
(supplied) |
Full ID of the bacteria isolated, incl. the target bacteria | 1 |
| JGKPT17
Sheep/Goats diseases |
3 Freeze dried cultures | BA01/SG – BA03/SG | 2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution | Conventional microbiology, API and auto systems | R.E.F 12.7
(supplied) |
Full ID of the bacteria isolated, incl. the target bacteria | 1 |
| JGKPT36-38
Trichomonas and Campylobacter ID by Culture |
5 Cultures in transport media | SW01 – SW05 | 2-8oC | NA
Ready to process |
Preferably immediately but no later than 5 days after dispatch | Conventional microbiology | R.E.F 61.4
(supplied) |
Presence/Absence: Trichomonas foetus, Campylobacter fetus | 1 |
| JGKPT37 & 39
Trichomonas and Campylobacter ID by PCR |
Molecular technique | Presence/Absence: Trichomonas foetus | 1 | ||||||
|
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
|
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FD: Freeze-Dried