PT Instructions
WEEK 09: 26 FEBRUARY 2024 RUN
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme Description and Code | Samples | Samples reconstitution | Testing timeframe | Approved test methods | Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| Antibiogram – mastitis pathogens
JGKPT02 |
2 x FD cultures
1 x QC plate, NA Plate with 2 grown bacteria Notes: 1. Culturing must be done from the FD cultures (see reconstitution) 2. The NA plate is only a QC plate, evidence that the FD cultures have been QC’ passed. |
ATB01/M – Dairy cow
ATB02/M – Dairy cow |
2-8oC | Allow cultures and sDH2O to adjust to room temperature.
Remove seals and bungs from the vials and add 300 µl sDH2O to each vial. Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per lab’s SOP). Using a sterile loop streak to facilitate discrete colonies |
These samples are not intended to be long-term storage.
Preferably, test the samples immediately upon receipt and upon reconstitution |
Culturing: Micro
Susceptibility: Disc diffusion (Kirby-Bauer) / Qualitative
|
R.E.F 02
(supplied) |
Zone Ø in millimeters.
Report results as: Sensitive (S), Intermediate (I), Resistance (R) |
Culture: 1 rep
Susceptibility: 3 replicates (supplied discs) |
| Antimicrobial discs
(x 18) |
Oxacillin 1 µg (x 6)/ Oxoid; Lot # 3646846; Exp. 03/2026
Pirlimycin 2 µg (x 6)/ Oxoid; Lot # 3777591; Exp. 01/2025 Penicillin/Novobiocin 40 µg (x 6)/ Oxoid; Lot # 3711351; Exp. 08/2024 |
Commercial discs:
Ready to use |
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| Antimicrobial residues – meat
JGKPT23-25 |
4 x Fresh samples
(chicken liver) |
RES-01-F – RES-04-F | 2-8oC | Fresh samples: Ready to process | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 23-25
(supplied) |
Presence/Absence of antimicrobials
ID of the antibiotic |
1 rep |
| Antimicrobial residues – milk
JGKPT20-22 |
4 x bovine milk | RES-01-M – RES-04-M | 2-8oC | N/A
Ready to process |
Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 20-22
(supplied) |
Presence/Absence of antimicrobials
Name of the antibiotic |
1 rep |
| Food hygiene – Detection and Enumeration
JGKPT26-30 |
Detection:
4 x fresh samples
Enumeration: 4 x FD samples |
Detection:
FH01/D – FH04/D
Enumeration: FH01 – FH04/Counts |
2-8oC | Detection:
Ready to process as per SOP
Enumeration: Allow samples to adjust to room temperature. Add 5 ml preheated (37oC) sterile DH2O to each jar. Mix content thoroughly on a vortex until there is no clumps. Process as per lab’s SOP |
Test immediately upon receipt/
reconstitution The FD samples are not intended for long-term storage |
Microbiology (conventional & PCR) | R.E.F 26-30
(supplied) |
Counts | Detection:
1 rep
Enumeration: TBC: 2 rep Coliforms: 2 rep E. coli: 2 rep |
| Mastitis pathogens
JGKPT34 |
2 x mastitis FD cultures | MAST01 – MAST02 | 2-8oC | Allow cultures and sDH2O to adjust to room temperature.
Remove seals and bungs from the vials and add 300 µl sDH2O to each vial. Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per lab’s SOP). Using a sterile loop streak to facilitate discrete colonies |
Test immediately upon receipt/
Reconstitution. These samples are not intended for long-term storage |
Culture & ID | R.E.F 33-34
(supplied) |
Target bacteria | 1 rep |
| Milk hygiene: TBC, Coliforms and E. coli counts
JGKPT17-19 |
4 x FD milk (bovine) | MH01 – MH04 | 2-8oC | Add 6 ml sDH2O to vials 1, 2, 4. Add 5 ml sDH2O to vial 3.
Notes: Do not use cold water. Samples must adjust to room temperature for ≥ 30 min before adding sD.H2O. D.H2O must be at 37oC in a water bath for ≥30 min before use. Mix content thoroughly on a vortex until there is no clumps. |
Test immediately upon receipt/
Reconstitution. These samples are not intended for long-term storage |
3M Petri Films
Conventional technique |
R.E.F 17-19 | Counts | TBC: 2 rep
Coliforms: 2 rep E. coli: 2 rep |
| Treatment of samples
No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests None
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 20th of March 2024. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None |
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FD: Freeze-Dried