PT Instructions

WEEK 44: 24 OCTOBER 2022 RUN

PURPOSE

To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1

INSTRUCTIONS

Scheme Description and Code Samples Samples  reconstitution Testing timeframe Approved test methods Sheet to be used for submission of results to JGK Unit of measurement (Results) Number of test replicates per sample
Type Identification

(as per the labels)

 Storage
Antibiogram – mastitis pathogens

JGKPT02

 2 x Cows’ milk ATB01M – ATB02M 2-8oC Milk samples: Ready to use; proceed with culturing Culturing of bacteria should be done Immediately upon receipt of the samples Culturing: Micro

Susceptibility: Kirby-Bauer / Disc diffusion (Qualitative)

 R.E.F 02

(supplied)

 Zone Ø in millimeters

(Results: S, I, R)

 Culture: 1 rep

Susceptibility:

3 replicates (supplied discs)
18 x Antibiotic discs 6 x Cephalexin 30 µg

6 x Norfloxacin 30 µg

6 x Penicillin G 10 IU

 Commercial discs:

Ready to use
2 x Clean Mueller Hinton agar plates Wrapped in aluminum foil Supplied MH agar plates:

ATB02/M susceptibility test must be conducted using the supplied MH agar plates
Antimicrobial residues – meat

JGKPT23-25

 4 x Fresh samples

(chicken muscle)

 RES-01-F – RES-04-F 2-8oC Fresh samples: Ready to process

 

FD samples: Add 10 ml sterile DH2O to each jar. NB: samples and water must first adjust to room To for ≥ 30 min

 Test immediately upon receipt/ reconstitution of the samples Rapid test

Microbiology

Chromatography

 R.E.F 23-25

(supplied)

 Presence/Absence of antimicrobials

 

ID of the antibiotic

 1 rep
4 x FD samples (chicken muscle)
Antimicrobial residues – milk

JGKPT20-22

 4 x FD milk (bovine) RES-01-M – RES-04-M 2-8oC Add 5 ml sterile DH2O

to each vial

Note: Do not use cold water. Samples and D.H2O must all adjust to room To before use. Preferably, use water at 37oC to obtain better recon result.

 Test immediately upon reconstitution Rapid test

Microbiology

Chromatography

 R.E.F 20-22

(supplied)

 Presence/Absence of antimicrobials

ID of the antibiotic

 1 rep
Food hygiene – meat (Detection)

JGKPT25-30

 4 x Fresh chicken muscles FH01 – FH04 2-8oC Ready to process Test immediately upon receipt

 

NB: all samples (FD and swabs) must be tested and results submitted separately

 Microbiology (conventional & PCR) R.E.F 25-30

(supplied)

 Presence/Absence of target bacteria FD: 1 rep

Swab: 1 rep
4 x FD samples Add 10 ml sterile DH2O

to each jar
4 x Amies swabs Backup samples for FH01 – FH0-4. Ready to process
Food hygiene – meat (Enumeration) 4 x FD samples FH01/Counts – FH04/Counts 2-8oC Add 20 ml preheated (37oC) sterile DH2O

to each jar

 Test immediately upon receipt/

reconstitution

 Counts TBC: 2 rep

Coliforms: 2 rep

E. coli: 2 rep
Mastitis pathogens – milk

JGKPT34

 2 x Cows’ milk MAST01 – MAST02 2-8oC Ready to process Test immediately upon receipt Culture & ID R.E.F 33-34

(supplied)

 Target bacteria 1 rep
Milk hygiene: TBC, Coliforms and E. coli counts

JGKPT17-19

 3 x FD milk (bovine) MH01 – MH03 (FD)

MH04 (Liquid)

 2-8oC Add 20 ml sterile DH2O

to each vial

Notes:

N.B: Do not use cold water. Samples must adjust to room To for ≥ 30 min before adding D.H2O.

D.H2O must be at 37oC in a water bath for ≥30 min before use

 Test immediately upon reconstitution 3M Petri Films

Conventional technique

 R.E.F 17-19 Counts TBC: 2 rep

Coliforms: 2 rep

E. coli: 2 rep
1 x Fresh milk (bovine) 2-8oC Do not freeze
Aflatoxins & Fumonisins

JGKPT31-32

 To follow To follow To follow To follow To follow To follow To follow To follow To follow
BVD & Fasciola

JGKPT39-40

 5 x FD sera (bovine) BVD/FAS01 – BVD/FAS05 2-8oC Samples 1, 2, 3, 5: Add 250 µL sterile DH2O to each vial.

Sample 4: Add 500 µL sterile DH2O to the vial

 

 Test immediately upon reconstitution ELISA R.E.F 39-45 Elisa ODs 2 rep.
Avian influenza - Abs

JGKPT41

 6 x FD sera (chicken) AI01 – AI06 2-8oC The volume is not the same for all the samples. The handwritten number on the vial represents the volume of the sterile D.H2O to be added to the sample. If it is written 350, add 350 sD.H2O to the vial Test immediately upon reconstitution ELISA

HI

 R.E.F 39-45 ODs / Titres 2 rep
Inf. bronchitis virus – Abs

  JGKPT42

 6 x FD sera (chicken) IBV01 – IBV06 2-8oC The volume is not the same for all the samples. The handwritten number on the vial represents the volume of the sterile D.H2O to be added to the sample. If it is written 350, add 350 sD.H2O to the vial Test immediately upon receipt ELISA

HI

 R.E.F 39-45 ODs / Titres 2 rep
Newcastle disease – Abs JGKPT43 6 x FD sera (poultry) NDV01 – NDV06 2-8oC The volume is not the same for all the samples. The handwritten number on the vial represents the volume of the sterile D.H2O to be added to the sample. If it is written 350, add 350 sD.H2O to the vial Test immediately upon reconstitution ELISA

HI

 R.E.F 39-45 ODs / Titres 2 rep
Salmonella enteritidis – Abs

JGKPT44

 6 x FD sera (poultry) SE01 – SE06 2-8oC The volume is not the same for all the samples. The handwritten number on the vial represents the volume of the sterile D.H2O to be added to the sample. If it is written 350, add 350 sD.H2O to the vial Test immediately upon receipt ELISA

RPAT

 R.E.F 39-45 Positive / Negative 1 rep
Mycoplasma gallisepticum - Abs

JGKPT45

 10 x FD sera (poultry) MG01 – MG10 2-8oC The volume is not the same for all the samples. The handwritten number on the vial represents the volume of the sterile D.H2O to be added to the sample. If it is written 350, add 350 sD.H2O to the vial Test immediately upon receipt ELISA

RPAT

 R.E.F 39-45

 

Use 2 separate result sheets:

Sheet 1: results for MG01 – MG06 (WK44 PT Report)

Sheet 2: results for MG07 – MG10

(WK20 PT Report)

 Positive / Negative 1 rep
Treatment of samples

No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.

 

Handling and safety requirements

Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.

 

Specific environmental conditions required for the participant to conduct tests

None

 

Results recording

Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.

 

Submission of PT results to JGK

All results must be submitted on or before the 9th of November 2022. Any results received after this date will not be included in the analysis.

 

Enquiries

For any inquiry, please use the contact details provided above to contact JGK

 

Return of the proficiency test items

None

FD: Freeze-Dried