25 & 26 JULY 2022 RUN – JGK Lab AfriQA

Scheme reference	Samples			Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
	Type	Identification (as per the labels)	Storage
JGKPT01 Antimicrobial susceptibility (Clin. Pathogens)	2 Cultures on NA plates + 1 Amies swab, BKP sample for ATB02/C	ATB01/C – equine ATB02/C – dog	2-8^oC	N/A Proceed with culture / subculture	Preferably, test immediately but no later than 48 hours from date of dispatch	Disc diffusion technique (Kirby Bauer)	R.E.F 1.0 (available on www.jgklab.co.za)	Zone Ø in millimeters. Results: Sensitive (S), Intermediate (I), Resistance (R)	Culture ID: 1 Susceptibility: Supplied discs: 3 (as per result sheet)
	Antimicrobial discs (x18)	Gentamycin 10 µg (x6) Doxycycline 30 µg (x6) Rifampicin 5 µg (x6)		Discs are ready to use (Commercial discs)
JGKPT03 Brucella: RBT (Samples for labs doing RBT only)	6 FD cattle sera 1 PC (101). 0.5 ml 1 NC (CANEG) 1 ml	RBT01 – RBT06	2-8^oC	Samples 1, 2, 4, 5: Add 1 ml sterile DH₂O to each vial Samples 3, 6 Add 0.5 ml sterile DH₂O to each vial	Once reconstituted, samples must be tested without delay	RBT only	R.E.F 3-5 (available on www.jgklab.co.za)	Positive/Negative Positive results must be scored 1+ to 3+	1
JGKPT04 Brucella: RBT/CFT (Samples for labs doing both RBT and CFT)	6 FD cattle sera 1 PC (T392) 1 ml 1 NC (CANEG) 1 ml	RBT/CFT01 – RBT/CFT06	2-8^oC	Samples 1, 3, 4, 6: Add 1 ml sterile DH₂O to each vial Sample 2: Add 0.5 ml sterile DH₂O to the vial Sample 5: Add 2 ml sterile DH₂O to the vial	Once reconstituted, samples must be tested without delay	RBT and CFT	R.E.F 3-5 (available on www.jgklab.co.za)	RBT: Positive/Negative Positive results must be scored 1+ to 3+	1
								CFT: CFTIU/ml	2
JGKPT05 Brucella: MRT	4 bulk tank milk	CAM01 – CAM04	2-8^oC	NA Ready to process	Test immediately upon receipt	MRT	R.E.F 3-5 (available on www.jgklab.co.za)	Positive/Negative	1
JGKPT06 Brucella: Culture & Identification	Samples 1 & 3: FD culture Samples 2 & 4: cow’s milk	CAC01 – CAC04	2-8^oC	Samples 1 and 3: Add 350 µL sterile D.H₂O to each vial Samples 2 and 4: ready to use	Once reconstituted, samples must be tested without delay	Conventional microbiology and Molecular technique	R.E.F 06 (available on www.jgklab.co.za)	Presence/Absence: Brucella abortus Strain: Field or vaccine	1
JGKPT07 Brucella Slides	6 unstained smears	Slide 1: x 2 Slide 2: x 2 Slide 3: x 2	Room T^o Dust-free	Proceed with staining technique as per Lab SOP	When and as convenient, but before submission deadline	Stamp’s staining technique	R.E.F 06 (available on www.jgklab.co.za)	Positive/Negative for Brucella	1
JGKPT08 Listeria species	3 FD cultures	LM01 – LM03	2-8^oC	Add 350 µL sterile DH₂O to each vial	Once reconstituted, samples must be tested without delay	Conventional microbiology	R.E.F 08-10 (available on www.jgklab.co.za)	Positive/Negative for Listeria + full ID of the listeria isolated	1
JGKPT09 Salmonella Poultry	3 Cultures on NA plate	SAP01 – SAP03	2-8^oC	N/A Ready to use	Test immediately upon receipt	Conventional microbiology and Molecular technique	R.E.F 08-10 (available on www.jgklab.co.za)	Positive/Negative for Salmonella + full ID of isolated salmonella	1
JGKPT09 Salmonella Mammals	3 Cultures on NA plate	SAM01 – SAM03							1
JGKPT13 Bacteriology: DOG & CAT	3 Cultures on NA plate + 1 x Amies swab and 2 x broth, as BKP samples	BAC01/DC – BAC03/DC BAC01/DC & BAC03/DC: BKP samples = broth in vials BAC02/DC: BKP sample = Amies swabs	2-8^oC	N/A Proceed with culture / subculture	Test immediately upon receipt	Conventional microbiology and Molecular technique	R.E.F 11-16 (available on www.jgklab.co.za)	Full ID of all bacteria isolated from each sample	1
JGKPT14 Bacteriology: HORSE	3 Cultures on NA plate + 3 x broth, as BKP samples	BAC01/H – BAC03/H BAC01/H – BAC03/H: BKP samples in vials	2-8^oC	N/A Proceed with culture / subculture	Test immediately upon receipt
JGKPT35 & 37 Trichomonas and Campylobacter ID by Culture	5 Cultures in transport media	SW01 – SW05	2-8^oC	NA Ready to process	Test immediately upon receipt	Conventional microbiology	R.E.F 35-38 (available on www.jgklab.co.za)	Presence/Absence: Trichomonas foetus, Campylobacter fetus	1
JGKPT36 & 38 Trichomonas and Campylobacter ID by PCR						Molecular technique		Presence/Absence: Trichomonas foetus	1
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory. Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company. Specific environmental conditions required for the participant to conduct tests JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+ Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The R.E.F grouped into WK8,19,31,43 and WK9,20,32,44 are available online (www.jgklab.co.za) and can be downloaded under “PUBLICATIONS” The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F. Submission of PT results to JGK All results must be submitted on Wednesday, 10^th of August 2022. Any results received after this date will not be included in the analysis. Enquiries For any inquiry, please use the contact details provided above to contact JGK Return of the proficiency test items None