PT Instructions
26 July 2021 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme
reference |
Samples | Samples reconstitution | Testing timeframe | Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| JGKPT01
Antimicrobial susceptibility (clin. pathogens) |
2 Broths | ATB01/C – pig
ATB02/C – chickens |
2-8oC | NA
Ready to process |
Preferably, test immediately upon receipt of the samples | Disc diffusion technique
(Kirby Bauer) |
R.E.F 1.0
(available on www.jgklab.co.za) |
Zone Ø in millimeters.
Results: Sensitive (S), Intermediate (I), Resistance (R) |
Culture ID: 1
Susceptibility: Supplied discs: 3 (as per result sheet) |
| Antimicrobial discs (x18) | Imipenem 10 µg (x6)
Rifampicin 10 µg (x6) Cefpodoxime 5 µg (x6) |
Discs are ready to use (Commercial discs) | |||||||
| JGKPT03
Brucella: RBT |
6 FD cattle sera
(samples for Labs testing for RBT only) |
RBT01 – RBT06 | 2-8oC | Add 0.5 ml sterile DH2O to each vial | Test immediately after reconstitution of the samples before deadline for submission of results | RBT | R.E.F 3-5
(available on www.jgklab.co.za) |
Positive/Negative
Agglutination score for positive results (1+ to 3+) |
1 |
| JGKPT04
Brucella: CFT |
6 FD cattle sera (samples for Labs testing for both RBT and CFT) | RBT/CFT01 – RBT/CFT06 | 2-8oC | Add sterile DH2O as follows:
0.5 ml to sample 2 1 ml to all other samples |
Test immediately after reconstitution of the samples before deadline for submission of results | RBT
CFT (DAFF approved SOP for SA Labs) |
R.E.F 3-5
(available on www.jgklab.co.za) |
RBT: Positive/Negative,
agglutination score for positive results (1+ to 3+) CFT: CFTIU/ml |
RBT: 1
CFT: 2 |
| JGKPT05
Brucella: MRT |
4 bulk tank milk | CAM01 – CAM04 | 2-8oC | NA
Ready to process |
Preferably, test immediately but no later than 48 hours from date of dispatch | MRT | R.E.F 3-5
(available on www.jgklab.co.za) |
Positive/Negative | 1 |
| JGKPT06
Brucella: Culture & Identification |
4 FD cultures | CAC01 – CAC04 | 2-8oC | Add 0.45 ml sterile DH2O to each vial | Test immediately after reconstitution of the samples before deadline for submission of results | Conventional microbiology and
Molecular technique |
R.E.F 06
(available on www.jgklab.co.za) |
Brucella abortus: Presence/Absence
Strain of BA isolated: Field or vaccine |
1 |
| JGKPT07
Brucella Slides |
3 unstained smears | Slide 1: x 3
Slide 2: x 3 Slide 3: x 3 |
Room To
Dust-free |
Proceed with staining technique as per Lab SOP | When and as convenient, but before submission deadline | Stamp’s staining technique | R.E.F 06
(available on www.jgklab.co.za) |
Positive/Negative for Brucella | 1 |
| JGKPT09
Salmonella Poultry |
3 Broths | SAP01 – SAP03 | 2-8oC | NA
Ready to process |
Preferably, test immediately upon receipt of the samples | Conventional microbiology and
Molecular technique |
R.E.F 08-10
(available on www.jgklab.co.za) |
Positive/Negative for Salmonella + full ID of target salmonella | 1 |
| JGKPT10
Salmonella mammals |
3 Broths | SAM01 – SAM03 | 2-8oC | NA
Ready to process |
Positive/Negative for Salmonella + full ID of target salmonella | 1 | |||
| JGKPT12
Chickens Disease Scenarios |
3 Broths | BAC01/CH – BAC03/CH | 2-8oC | NA
Ready to process |
Preferably, test immediately upon receipt of the samples | Conventional microbiology and
Molecular technique |
R.E.F 11-16
(available on www.jgklab.co.za) |
Full ID of all the bacteria isolated in each sample | 1 |
| JGKPT15
Pig Disease Scenarios |
3 Broths | BAC01/P – BAC03/P | 2-8oC | NA
Ready to process |
1 | ||||
| JGKPT35 & 37
Trichomonas and Campylobacter ID by Culture |
5 Cultures in transport media | SW01 – SW05 | 2-8oC | NA
Ready to process |
Preferably, test immediately but no later than 72 hours from date of dispatch | Conventional microbiology | R.E.F 35-38
(available on www.jgklab.co.za) |
Presence/Absence: Trichomonas foetus, Campylobacter fetus | 1 |
| JGKPT36 & 38
Trichomonas and Campylobacter ID by PCR |
Molecular technique | Presence/Absence: Trichomonas foetus | 1 | ||||||
| Treatment of samples
No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on Wednesday, 11th of August 2021. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
|
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FD: Freeze-Dried