PT Instructions
28 July 2020 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme
reference |
Samples | Samples reconstitution | Testing timeframe | Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| JGKPT01
Antimicrobial susceptibility (Clin. pathogens) |
2 FD cultures | ATB01/C – Dog
ATB02/C – Pig |
2-8oC | Add 1 ml sterile DH2O to the vial. Mix | Test without delay after reconstitution of the samples | Disc diffusion technique (Qualitative - Kirby Bauer) | R.E.F 1.0
(supplied) |
Zone Ø in millimeters.
Results: Sensitive (S), Intermediate (I), Resistance (R) |
Culture ID: 1
Susceptibility: Supplied discs: 3 (as per result sheet) |
| Antimicrobial discs (x18) | Chloramphenicol 30 µg (6)
Gentamycin 10 µg (6) Polymixin B 300 units (6) |
Discs are ready to use (Commercial discs) | |||||||
| JGKPT03
Brucella: RBT |
6 FD cattle sera
(samples for Labs testing for RBT only) |
RBT01 – RBT06 | 2-8oC | Add 0.5 ml sterile DH2O to the vial. Mix well before testing | Test without delay after reconstitution of the samples | RBT | R.E.F 3-5
(supplied) |
Positive/Negative | 1 |
| JGKPT04
Brucella: CFT |
6 FD cattle sera (samples for Labs testing for both RBT and CFT) | RBT/CFT01 – RBT/CFT06 | 2-8oC | Sample 2: Add 0.5 ml sterile DH2O to the vial. All the other samples, add 1 ml. Mix well | Test without delay after reconstitution of the samples | CFT
(DAFF approved SOP for SA Labs) |
R.E.F 3-5
(supplied) |
CFTIU/ml | 2 |
| JGKPT05
Brucella: MRT |
4 bulk tank milk | CAM01 – CAM04 | 2-8oC | NA
Ready to process |
Preferably immediately but no later than 3 days from date of dispatch | MRT | R.E.F 3-5
(supplied) |
Positive/Negative | 1 |
| JGKPT06
Brucella ID: Culture & PCR |
2 FD cultures
2 milk |
CAC01 & CAC04: Milk
CAC02 & CAC03: FD |
2-8oC | FD: Add 1 ml sterile DH2O to the vial. Mix well before testing
Milk: Ready to process |
FD: Test without delay after reconstitution of the samples | Conventional microbiology and
Molecular technique |
R.E.F 06
(supplied) |
Presence/Absence: Brucella abortus
Strain: Field or vaccine |
1 |
| JGKPT07
Brucella Slides |
3 unstained smears | Slide 1: x 3
Slide 2: x 3 Slide 3: x 3 |
Room To
Dust-free |
Proceed with staining technique as per Lab SOP | When and as convenient, but before submission deadline | Stamp’s staining technique | R.E.F 06
(supplied) |
Positive/Negative for Brucella | 1 |
| JGKPT09
Salmonella Poultry |
2 FD cultures
1 Swab |
SAP01 & SAP03: FD
SAP02: Swab |
2-8oC | Add 0.5 ml sterile DH2O to the vial. Mix well | Test without delay after reconstitution of the samples | Conventional microbiology and
Molecular technique |
R.E.F 08-10
(supplied) |
Positive/Negative for Salmonella + full ID of target salmonella | 1 |
| JGKPT10
Salmonella mammals |
3 FD cultures | SAM01 – SAM03 | 2-8oC | Add 0.5 ml sterile DH2O to the vial. Mix well | Positive/Negative for Salmonella + full ID of target salmonella | 1 | |||
| JGKPT11
Diseases of Chicken |
3 FD cultures | BAC01/CH – BAC03/CH | 2-8oC | Add 0.5 ml sterile DH2O to the vial. Mix well | Test immediately upon reconstitution of the samples | Conventional microbiology and
Molecular technique |
R.E.F 08-10
(supplied) |
Full ID of the bacteria isolated | 1 |
| JGKPT35 & 37
Trichomonas and Campylobacter ID by Culture |
5 Cultures in transport media | SW01 – SW05 | 2-8oC | NA
Ready to process |
Preferably immediately but no later than Friday 31st July for culture (samples were prepared on Sunday 26 July) | Conventional microbiology | R.E.F 35-38
(supplied) |
Presence/Absence: Trichomonas foetus, Campylobacter fetus | 1 |
| JGKPT36 & 38
Trichomonas and Campylobacter ID by PCR |
Molecular technique | Presence/Absence: Trichomonas foetus | 1 | ||||||
|
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on 12th of August 2020. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
|
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FD: Freeze-Dried