PT Instructions
28 October 2019 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
Deadline for Submission of results: 20 November 2019
Results received after this date will not be included in the analysis.
| Scheme
reference |
Samples | Samples reconstitution | Testing timeframe | Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| JGKPT01
Antimicrobial susceptibility (clin. pathogens) |
Animal sp.: (1) Pig and (2) Dog
2 FD cultures |
ATB01/C
ATB02/C |
2-8oC | Add 1 ml sterile DH2O to the vial | Test immediately after reconstitution | Disc diffusion technique | R.E.F 1.2
(supplied) |
Zone Ø in millimeters.
Results: Sensitive (S), Intermediate (I), Resistance (R) |
Culture ID: 1
Susceptibility: Supplied discs: 3 (as per result sheet) |
| Antimicrobial discs (x18) | Gentamycin 30 µg (x6)
Penicillin 10 µg (x6) Sulphamethoxazole 100 µg (x6) |
Discs are ready to use (Commercial discs) | |||||||
| JGKPT03
Brucella: RBT (for RBT only) |
6 FD cattle sera | RBT01 – RBT06 | 2-8oC | Add 1 ml sterile DH2O to the vial | Test immediately after reconstitution | RBT | R.E.F 3.5
(supplied) |
RBT: Positive/Negative CFT: CFTIU/ml |
1 |
| JGKPT04
Brucella: CFT (for RBT & CFT) |
6 FD cattle sera | RBT/CFT01 – RBT/CFT06 | 2-8oC | Add 1 ml sterile DH2O to the vial | Test immediately after reconstitution | CFT
(DAFF approved SOP for SA Labs) |
R.E.F 3.5
(supplied) |
2 | |
| JGKPT05
Brucella: MRT |
4 cow’s milk | CAM01 – CAM04 | 2-8oC | NA
Ready to process |
Preferably immediately upon receipt | MRT | R.E.F 3.5
(supplied) |
Positive/Negative | 1 |
| JGKPT06
Brucella: Culture & Identification |
2 Freeze dried cultures
1 milk (20 ml) |
CAC01 – CAC03 | 2-8oC | Add 1 ml sterile DH2O to the vial
The milk is ready to use |
Test immediately after reconstitution/receipt (milk) | Conventional microbiology and
Molecular technique |
R.E.F 06
(supplied) |
Presence/Absence: Brucella abortus
Strain: Field or vaccine |
1 |
| JGKPT07
Brucella Slides |
3 unstained smears | Slide 1 - Slide 3 (x 3 each) | Room To
Dust-free |
Proceed with staining technique as per Lab SOP | When and as convenient, but before submission deadline | Stamp’s staining technique | R.E.F 06
(supplied) |
Positive/Negative for Brucella | 1 |
| JGKPT36-38
Trichomonas and Campylobacter ID by Culture |
5 Cultures in transport media | SW01 – SW05 | 2-8oC | NA
Ready to process |
Preferably immediately but no later than 5 days after dispatch | Conventional microbiology | R.E.F 61.4
(supplied) |
Presence/Absence: Trichomonas foetus, Campylobacter fetus | 1 |
| JGKPT37 & 39
Trichomonas and Campylobacter ID by PCR |
Molecular technique | Presence/Absence: Trichomonas foetus | 1 | ||||||
|
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
|
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FD: Freeze-Dried