PT Instructions
WEEK 44: 28 & 29 OCTOBER 2024 RUN
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme Description and Code | Samples | Samples reconstitution | Testing timeframe | Approved test methods | Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| Antibiogram – Mastitis pathogens
JGKPT02 |
2 x FD cultures | ATB01/M – Dairy cow
ATB02/M – Dairy cow |
2-8oC | Allow cultures and sDH2O to adjust to room temperature.
Remove seals and bungs from the vials and add 400 µl sDH2O to each vial. Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per your lab’s SOP). Using a sterile loop streak to facilitate discrete colonies |
These samples are not intended to be stored.
Test the samples immediately upon receipt and upon reconstitution |
Culturing: Micro
Susceptibility: Disc diffusion (Kirby-Bauer) / Qualitative
|
R.E.F 02
(supplied) |
Zone Ø in millimeters.
Report results as: Sensitive (S), Intermediate (I), Resistance (R) |
Culture: 1 rep
Susceptibility: 3 replicates (supplied discs) |
| Antimicrobial discs
(x 18+) |
Ceftiofur 30 µg; Oxoid Lot # 3690509; exp. 07/2024
Oxacillin 1 µg; Oxoid Lot # 3646846; Exp. 27/03/2026 Penicillin/Novobiocin 40; Oxoid Lot # 3711351; Exp. 08/2024 |
Commercial discs:
Ready to use |
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| Antimicrobial residues – Meat
JGKPT23-25 |
4 x Fresh samples
(chicken liver) |
RES-01/F – RES-04/F | 2-8oC | Fresh samples: Ready to process | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 23-25
(supplied) |
Presence/Absence of antimicrobials
ID of the antibiotic |
1 rep |
| Antimicrobial residues – Milk
JGKPT20-22 |
4 x FD bovine bulk milk | RES-01 – RES-04 | 2-8oC | Allow samples to adjust to room temperature. Add 4 ml preheated (37oC) sterile DH2O to each vial. Mix content thoroughly on a vortex until there is no clumps. | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 20-22
(supplied) |
Presence/Absence of antimicrobials
Name of the antibiotic |
1 rep |
| Food hygiene – Detection
JGKPT25-30 |
4 x Amies swabs | FH01 – FH04 | 2-8oC | N/A
Ready to process |
Test immediately upon receipt | Microbiology (conventional & PCR) | R.E.F 25-30
(supplied) |
ID of bacterial isolate or presence/absence of the target bacteria | 1 rep |
| Food hygiene – Enumeration | 4 x FD samples | FH01/Counts – FH04/Counts | 2-8oC | Allow samples to adjust to room temperature. Add 5 ml preheated (37oC) sterile DH2O to each jar. Mix content thoroughly on a vortex until there is no clumps | These samples are not intended to be stored.
Test the samples immediately upon receipt and upon reconstitution |
Counts | TBC: 2 rep
Coliforms: 2 rep E. coli: 2 rep |
||
| Mastitis pathogens JGKPT34 | 2 x FD cultures | MAST01 – MAST02 | 2-8oC | Allow cultures and sDH2O to adjust to room temperature.
Remove seals and bungs from the vials and add 400 µl sDH2O to each vial. Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per your lab’s SOP). Using a sterile loop streak to facilitate discrete colonies |
These samples are not intended to be stored.
Test the samples immediately upon receipt and upon reconstitution |
Culture & ID | R.E.F 33-34
(supplied) |
Target bacteria | 1 rep |
| Milk hygiene: TBC, Coliforms and E. coli counts
JGKPT17-19 |
6 x FD milk (bovine) | MH01 – MH06 | 2-8oC | Add 6 ml sterile DH2O
to each vial Notes: Do not use cold water. Samples must adjust to room temperature for ≥ 30 min before adding sD.H2O. D.H2O must be at 37oC in a water bath for ≥30 min before use. Mix content thoroughly on a vortex until there is no clumps. |
These samples are not intended to be stored.
Test the samples immediately upon receipt and upon reconstitution |
3M Petri Films
Conventional technique |
R.E.F 17-19 | Counts | TBC: 2 rep
Coliforms: 2 rep E. coli: 2 rep |
| BVD & Fasciola
JGKPT39-40 |
5 x FD sera (bovine) | BVD/FAS01 – BVD/FAS05 | 2-8oC | Allow samples and sDH2O to adjust to room temperature. Add 300 µL sDH2O to each vial. | Test immediately upon receipt/
reconstitution |
ELISA | R.E.F 39-45 | Elisa ODs | 2 rep. |
| Avian influenza - Abs
JGKPT41 |
6 x FD sera (chicken) | AI01 – AI06 | 2-8oC | Allow samples and sDH2O to adjust to room temperature. Sample volumes: ELISA only, left volume; ELISA & HI, right volume.
Add sDH2O as follows: Sample 1: 200/500 µL Sample 2: 300/500 µL Sample 3: 350/350 µL Sample 4: 200/500 µL Sample 5: 300/500 µL Sample 6: 200/500 µL |
Test immediately upon receipt/
reconstitution |
ELISA
HA/HI |
R.E.F 39-45 | ODs
Titres |
2 rep |
| Infectious bronchitis virus – Abs
JGKPT42 |
6 x FD sera (chicken) | IBV01 – IBV06 | 2-8oC | Allow samples and sDH2O to adjust to room temperature. Add sDH2O to the vials as follows:
Sample 1: 200 µL Sample 2: 300 µL Sample 3: 250 µL Sample 4: 300 µL Sample 5: 300 µL Sample 6: 300 µL |
Test immediately upon receipt/
reconstitution |
ELISA
HA/HI |
R.E.F 39-45 | ODs
Titres |
2 rep |
| Newcastle disease – Abs JGKPT43 | 6 x FD sera (poultry) | NDV01 – NDV06 | 2-8oC | Allow samples and sDH2O to adjust to room temperature. Add sDH2O to the vials as follows:
Sample 1: 300 µL Sample 2: 300 µL Sample 3: 300 µL Sample 4: 200 µL Sample 5: 300 µL Sample 6: 250 µL |
Test immediately upon receipt/
reconstitution |
ELISA
HA/HI |
R.E.F 39-45 | ODs
Titres |
2 rep |
| Salmonella enteritidis – Abs
JGKPT44 |
6 x FD sera (poultry) | SE01 – SE06 | 2-8oC | Allow samples and sDH2O to adjust to room temperature. Add sDH2O to the vials as follows:
Sample 1: 200 µL Sample 2: 200 µL Sample 3: 350 µL Sample 4: 300 µL Sample 5: 350 µL Sample 6: 300 µL |
Test immediately upon receipt/
reconstitution |
ELISA
RPAT |
R.E.F 39-45 | Positive / Negative | 1 rep |
| Mycoplasma gallisepticum - Abs
JGKPT45 |
6 x FD sera (poultry) | MG01 – MG06 | 2-8oC | Allow samples and sDH2O to adjust to room temperature. Add sDH2O to the vials as follows:
Sample 1: 300 µL Sample 2: 300 µL Sample 3: 200 µL Sample 4: 250 µL Sample 5: 300 µL Sample 6: 300 µL |
Test immediately upon receipt/
reconstitution |
ELISA
RPAT |
R.E.F 39-45 | Positive / Negative | 1 rep |
| Treatment of samples
No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests None
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before Wednesday the 20th of November 2024. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None |
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FD: Freeze-Dried