WEEK 32: 07 AUGUST 2023 RUN – JGK Lab AfriQA

Scheme Description and Code	Samples			Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
Scheme Description and Code	Type	Identification (as per the labels)	Storage	Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
Antibiogram – mastitis pathogens JGKPT02	2 x Cultures on NA plates All cultures are 1^st Generation/ Passage Sub- cultures	ATB01M – ATB02M	2-8^oC	Ready to use; proceed with culturing	Culturing of bacteria should be done Immediately upon receipt of the samples	Culturing: Micro Susceptibility: Kirby-Bauer / Disc diffusion (Qualitative)	R.E.F 02 (supplied)	Zone Ø in millimeters (Results: S, I, R)	Culture: 1 rep Susceptibility: 3 replicates (supplied discs)
Antibiogram – mastitis pathogens JGKPT02	21 x Antibiotic discs	7 x Ticarcillin 75 µg 7 x Tetracycline 30 µg 7 x Cephazolin 30 µg	2-8^oC	Commercial discs: Ready to use			R.E.F 02 (supplied)	Zone Ø in millimeters (Results: S, I, R)
Antimicrobial residues – meat JGKPT23-25	4 x Fresh samples (chicken liver)	RES-01-F – RES-04-F	2-8^oC	Fresh samples: Ready to process	Test immediately upon receipt of the samples	Rapid test Microbiology Chromatography	R.E.F 23-25 (supplied)	Presence/Absence of antimicrobials ID of the antibiotic	1 rep
Antimicrobial residues – milk JGKPT20-22	4 x Cows’ milk	RES-01-M – RES-04-M	2-8^oC	Ready to process	Test immediately upon receipt	Rapid test Microbiology Chromatography	R.E.F 20-22 (supplied)	Presence/Absence of antimicrobials ID of the antibiotic	1 rep
Food hygiene – VPH (DETECTION) JGKPT25-30	4 x Cultures on NA plates 4 x Amies swabs (BKP samples) All cultures are 1^st Generation/ Passage Sub- cultures	FH01 – FH04 (Swab samples serve to mimic abattoir hygiene monitoring)	2-8^oC	Ready to process	Test immediately upon receipt	Microbiology (conventional & PCR)	R.E.F 25-30 (supplied)	Presence/Absence of target bacteria	1 rep
Food hygiene – VPH (ENUMERATION)	4 x FD samples	FH01/Counts – FH04/Counts	2-8^oC	Samples must first adjust to room T^o Add 5 ml incubator-preheated (37^oC) sterile DH₂O to each jar Mix thoroughly to remove all clumps Do not add cold water	Test immediately upon receipt/ reconstitution	Microbiology (conventional & PCR)	R.E.F 25-30 (supplied)	Counts (cfu/ml)	TBC: 2 rep Coliforms: 2 rep E. coli: 2 rep
Mastitis pathogens – milk JGKPT34	2 x Cows’ milk	MAST01 – MAST02	2-8^oC	Ready to process	Test immediately upon receipt	Culture & ID	R.E.F 33-34 (supplied)	Target bacteria	1 rep
Milk hygiene: TBC, Coliforms and E. coli counts JGKPT17-19	4 x FD milk (bovine)	MH01 –MH04	2-8^oC	Add 5 ml sterile DH₂O to each jar Notes: N.B: Do not use cold water. Samples must adjust to room T^o for ≥ 30 min before adding D.H₂O. D.H₂O must be at 37^oC in a water bath/incubator for ≥30 min before use Mix thoroughly to remove all clumps	Test immediately upon reconstitution	3M Petri Films Conventional technique Read all Petri films (AC & COL/EC) after 48 hours of incubation	R.E.F 17-19	Counts	TBC: 2 rep Coliforms: 2 rep E. coli: 2 rep
Aflatoxins & Fumonisins JGKPT31-32	3 x maize meal	MYC01 – MYC03	2-8^oC	N/A Ready to process	Test immediately upon receipt	Chromatography	R.E.F 31-32	Detection & quantification of Aflatoxins (ppb) and Fumonisins (ppm)	1 rep.
Treatment of samples No special treatment should be given to the PT samples. They [PT samples] should be handled in the same manner as all the other samples routinely tested in the laboratory, including TAT Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company. Specific environmental conditions required for the participant to conduct tests None Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F. Submission of PT results to JGK All results must be submitted on or before Wednesday the 30^th of August 2023. Any results received after this date will not be included in the analysis. Enquiries For any inquiry, please use the contact details provided above to contact JGK Return of the proficiency test items None