PT Instructions
WEEK 19: 08 & 09 MAY 2023 RUN
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme
reference |
Samples | Samples reconstitution | Testing timeframe | Approved test methods | Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| JGKPT01
Antimicrobial susceptibility (Clin. Pathogens) |
2 x Grown Bacteria on NA Plates (main samples)
2 x Cultures on Amies swabs (BKP samples) NB: the bacteria on NA plates and on the swabs are identical |
ATB01/C – cattle
ATB02/C – chickens |
2-8oC | ATB01/C: Ready to use
ATB02/C: Ready to use |
Test the samples immediately upon receipt | Disc diffusion technique
(Kirby Bauer) |
R.E.F 1.0
(available on www.jgklab.co.za) |
Zone Ø in millimeters.
Report results as: Sensitive (S), Intermediate (I), Resistance (R) |
Culture ID: 1
Susceptibility: Supplied discs: 3 (as per result sheet) |
| Antimicrobial discs (x 21) | Cephazolin 30 µg (x 7)/ Oxoid; Lot # 3195880; Exp. 09/12/2023
Gentamycin 10 µg (x 7)/ Oxoid; Lot # 3108138; Exp. 30/07/2023 Tetracycline 30 µg (x 7)/ Oxoid; Lot # 3214586; Exp. 14/01/2024 |
Discs are ready to use (Commercial discs) | |||||||
| JGKPT03
Brucella: RBT (Samples for labs doing RBT only) |
6 FD cattle sera | RBT01 – RBT06 | 2-8oC | Samples 1, 2, 4 and 5:
Add 500 µl sDH2O to the vial
Sample 3: Add 1 ml sDH2O to the vial Sample 6: Add 250 µl sDH2O to the vial |
Once reconstituted, samples must be tested without delay | RBT only | R.E.F 3-5
(available on www.jgklab.co.za) |
Positive/Negative | 1 |
| JGKPT04
Brucella: RBT/CFT (Samples for labs doing both RBT and CFT) |
6 FD cattle sera | RBT/CFT01 – RBT/CFT06 | 2-8oC | Sample 1:
Add 2 ml sDH2O to the vial
Samples 2, 4, and 5: Add 500 µl sDH2O to the vial
Sample 3: Add 1 ml sDH2O to the vial
Sample 6: Add 700 µl sDH2O to the vial |
Once reconstituted, samples must be tested without delay | RBT and CFT | R.E.F 3-5
(available on www.jgklab.co.za) |
RBT: Positive/Negative | 1 |
| CFT:
Option 1: Endpoint reaction (e.g. 1/4 ++++, 1/128+)
Option 2: Titer (e.g. 24, 480)
NB: the corresponding serum dilution (e.g. 1/4) must always be indicated or ticked on the result sheet |
2
|
||||||||
| JGKPT05
Brucella: MRT |
4 bulk tank milk
(not preserved) |
CAM01 – CAM04 | 2-8oC | NA
Ready to process |
Test immediately upon receipt | MRT | R.E.F 3-5
(available on www.jgklab.co.za) |
Positive/Negative | 1 |
| JGKPT06
Brucella: Culture & Identification |
CAC01:
Amies swab CAC02: FD culture CAC03: Bovine milk CAC04: Amies swab |
CAC01 – CAC04 | 2-8oC | FD cultures:
Add 1 ml sD.H2O to each vial
Swabs: Ready to process
Milk: Ready to process |
Once reconstituted, sample must be tested without delay | Conventional microbiology and
Molecular technique |
R.E.F 06
(available on www.jgklab.co.za) |
Presence/Absence: Brucella abortus
Strain: Field or vaccine |
1 |
| JGKPT07
Brucella Slides |
6 unstained smears | Slide 1: x 2
Slide 2: x 2 Slide 3: x 2 |
Room To
Dust-free |
Proceed with staining technique as per Lab SOP | Test immediately upon receipt | Stamp’s staining technique | R.E.F 06
(available on www.jgklab.co.za) |
Positive/Negative for Brucella | 1 |
| JGKPT11
Bacteriology: Diseases of cattle |
Sample 1 (BA01/C):
Grown Bacteria on NA Plate (main sample) + Amies swab (BKP) Sample 2 (BA02/C): Grown Bacteria on NA Plate (main sample) + Amies swab (BKP) Sample 3 (BA03/C): Grown Bacteria on NA Plate (main sample) + Amies swab (BKP) Sample 4 (BA04/C): Bovine quarter milk
NB: the bacteria on NA plates and on the swabs are identical |
BA01/C – BA04/C
Disease scenarios available on a separate document |
2-8oC | NA plates:
Ready to process
Amies swabs: Ready to process
Milk: Ready to process
|
Test immediately upon receipt | Conventional microbiology and
Molecular technique |
R.E.F 11-16
(available on www.jgklab.co.za) |
Full bacterial identification | 1 |
| JGKPT12
Bacteriology: Diseases of chickens |
Sample 1 (BA01/CH):
Grown Bacteria on NA Plate (main sample) + Amies swab (BKP) Sample 2 (BA02/CH): Grown Bacteria on NA Plate (main sample) + Amies swab (BKP) Sample 3 (BA03/CH): Grown Bacteria on NA Plate (main sample) + Amies swab (BKP) NB: the bacteria on NA plates and on the swabs are identical |
BA01/CH – BA03/CH
Disease scenarios available on a separate document |
2-8oC | NA plates:
Ready to process
Amies swabs (BKP): Ready to process if necessary
|
Test immediately upon receipt | Conventional microbiology and
Molecular technique |
R.E.F 11-16
(available on www.jgklab.co.za) |
Full bacterial identification | 1 |
| JGKPT35 & 37
Trichomonas and Campylobacter ID by Culture |
5 Cultures in transport media | SW01 – SW05 | 2-8oC | Ready to process | Test immediately upon receipt | Conventional microbiology | R.E.F 35-38
(available on www.jgklab.co.za) |
Presence/Absence: Trichomonas foetus, Campylobacter fetus | 1 |
| JGKPT36 & 38
Trichomonas and Campylobacter ID by PCR |
Molecular technique | 1 | |||||||
|
Treatment of samples No special treatment should be given to the PT samples. They [PT samples] should be handled in the same manner as all the other samples routinely tested in the laboratory, including TAT
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The R.E.F grouped into WK8,19,31,43 and WK9,20,32,44 are available online (www.jgklab.co.za) and can be downloaded under “PUBLICATIONS” The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on Wednesday, 31st of May 2023. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
|
|||||||||
FD: Freeze-Dried