PT Instructions

01 & 02 AUGUST 2022 RUN

PURPOSE

To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1

INSTRUCTIONS

Scheme Description and Code Samples Samples  reconstitution Testing timeframe Approved test methods Sheet to be used for submission of results to JGK Unit of measurement (Results) Number of test replicates per sample
Type Identification

(as per the labels)

 Storage
Antibiogram – mastitis pathogens

JGKPT02

 2 x Cows’ milk

Plus

1 x NA Plate Backup sample

 ATB01M – ATB02M 2-8oC Cows’ milk and Agar Plate: Ready to use; proceed with subculturing

N.B: Main samples for ATB are Cows’ milk. Use BKP samples only if there is any challenge

 Culturing of bacteria should be done Immediately upon receipt of the samples Culturing: micro

Susceptibility: Kirby-Bauer / Disc diffusion (Qualitative)

 R.E.F 02

(supplied)

 Zone Ø in millimeters

(Results: S, I, R)

 Culture: 1 rep

Susceptibility:

3 replicates (supplied discs)
18 x Antibiotic discs 6 x Cefpodoxime 10 µg

6 x Penicillin 10 IU

6 x Sulphametoxazole 100 µg

 Commercial discs:

Ready to process
Antimicrobial residues – meat

JGKPT23-25

 4 x Meat pieces (chicken liver) RES-01-F – RES-04-F (fresh)

RES-01-F – RES-04-F (FD)

Process what you receive

 2-8oC Fresh liver: Ready to process

FD liver: Add 10 ml sterile DH2O to each jar

 Test immediately upon receipt/ reconstitution Rapid test

Microbiology

Chromatography

 R.E.F 23-25

(supplied)

 Presence/Absence of antimicrobials

ID of the antibiotic

 1 rep
Antimicrobial residues – milk

JGKPT20-22

 4 x FD milk (bovine) RES-01-M – RES-04-M (liquid)

RES-01-M – RES-04-M (FD)

Process what you receive

 2-8oC Liquid milk: Ready to process

FD milk: Add 5 ml sterile DH2O to each vial

Note: samples and D.H2O must all adjust to room To before use

 Test immediately upon receipt/ reconstitution Rapid test

Microbiology

Chromatography

 R.E.F 20-22

(supplied)

 Presence/Absence of antimicrobials

ID of the antibiotic

 1 rep
Food hygiene – meat (Detection)

JGKPT25-30

 4 x Fresh chicken livers FH01(D) – FH04(D)

D = Detection)

 2-8oC Ready to process Test immediately upon receipt Microbiology (conventional & PCR) R.E.F 25-30

(supplied)

 Presence/Absence of target bacteria 1 rep
Food hygiene – meat (Enumeration: TBC, Coliforms, E. coli)

JGKPT25-30

 4 x FD samples FH01(C) – FH04(C)

C = Counts

 2-8oC Add 10 ml sterile D.H2O to samples 1, 2, 3 (vials).

Add 20 ml sterile D.H2O to sample 4 (jar)

 Test immediately upon receipt/ reconstitution Microbiology (conventional & PCR) R.E.F 25-30

(supplied)

 Presence/Absence of target bacteria 1 rep
Mastitis pathogens – milk

JGKPT34

 2 x Fresh milk (bovine) MAST01 – MAST02 2-8oC Ready to process Test immediately upon receipt Culture & ID R.E.F 33-34

(supplied)

 Target bacteria 1 rep
Milk hygiene:

TBC, Coliforms and E. coli counts

JGKPT17-19

 4 x FD milk (bovine) MH01 – MH04 2-8oC Samples 1, 2, 3:

Add 10 ml sterile DH2O

to each vial

sample 4:

Add 20 ml sterile DH2O

to the jar

Notes:

Samples must adjust to room To for ≥ 30 min before adding D.H2O.

D.H2O must be at 37oC in water bath for ≥30 min before use

 Test immediately upon reconstitution 3M Petri Films

Conventional technique

 R.E.F 17-19 Counts 2 rep
Aflatoxins & Fumonisins

JGKPT31-32

 To follow To follow To follow To follow To follow To follow To follow To follow To follow
Treatment of samples

No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.

 

Handling and safety requirements

Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.

 

Specific environmental conditions required for the participant to conduct tests

None

 

Results recording

Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.

 

Submission of PT results to JGK

All results must be submitted on or before the 17th of August 2022. Any results received after this date will not be included in the analysis.

 

Enquiries

For any inquiry, please use the contact details provided above to contact JGK

 

Return of the proficiency test items

None

FD: Freeze-Dried