PT Instructions
09 & 10 May 2022 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme Description and Code | Samples | Samples reconstitution | Testing timeframe | Approved test methods | Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| Antibiogram – mastitis pathogens
JGKPT02 |
2 x Cultures on Nutrient Agar Plate | ATB01M – ATB02M | 2-8oC | Agar Plate: Ready to use; proceed with subculturing | Culturing of bacteria should be done Immediately upon receipt of the samples | Culturing: micro
Susceptibility: Kirby-Bauer / Disc diffusion (Qualitative) |
R.E.F 02
(supplied) |
Zone Ø in millimeters
(Results: S, I, R) |
Culture: 1 rep
Susceptibility: 3 replicates (supplied discs) |
| 18 x Antibiotic discs | 6 x Cephalexin 30 µg
6 x Tetracycline 30 µg 6 x Florfenicol 30 µg |
Commercial discs:
Ready to process |
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| Antimicrobial residues – meat
JGKPT23-25 |
4 x Meat pieces (chicken liver) | RES-01-F – RES-04-F | 2-8oC | Fresh liver: Ready to process
FD liver: Add 10 ml sterile DH2O to each jar |
Test immediately upon receipt/ reconstitution | Rapid test
Microbiology Chromatography |
R.E.F 23-25
(supplied) |
Presence/Absence of antimicrobials
ID of the antibiotic |
1 rep |
| Antimicrobial residues – milk
JGKPT20-22 |
4 x FD milk (bovine) | RES-01-M – RES-04-M | 2-8oC | Add 5 ml sterile DH2O
to each vial Note: samples and D.H2O must all adjust to room To before use |
Test immediately upon reconstitution | Rapid test
Microbiology Chromatography |
R.E.F 20-22
(supplied) |
Presence/Absence of antimicrobials
ID of the antibiotic |
1 rep |
| Food hygiene – meat (Detection)
JGKPT25-30 |
4 x Fresh chicken livers | FH01 – FH04
|
2-8oC | Ready to process | Test immediately upon receipt | Microbiology (conventional & PCR) | R.E.F 25-30
(supplied) |
Presence/Absence of target bacteria | 1 rep |
| Mastitis pathogens – milk
JGKPT34 |
2 x Fresh milk (bovine) | MAST01 – MAST02 | 2-8oC | Ready to process | Test immediately upon receipt | Culture & ID | R.E.F 33-34
(supplied) |
Target bacteria | 1 rep |
| Milk hygiene: TBC, Coliforms and E. coli counts
JGKPT17-19 |
4 x FD milk (bovine) | MH01 – MH04 | 2-8oC | Add 10 ml sterile DH2O
to each vial Notes: Samples must adjust to room To for ≥ 30 min before adding D.H2O. D.H2O must be at 37oC in water bath for ≥30 min before use |
Test immediately upon reconstitution | 3M Petri Films
Conventional technique |
R.E.F 17-19 | Counts | 2 rep |
| Aflatoxins & Fumonisins
JGKPT31-32 |
To follow | To follow | To follow | To follow | To follow | To follow | To follow | To follow | To follow |
| BVD & Fasciola
JGKPT39-40 |
5 x FD sera (bovine) | BVD/FAS01 – BVD/FAS05 | 2-8oC | Add 300 µL sterile DH2O to each vial | Test immediately upon reconstitution | ELISA | R.E.F 39-45 | Elisa ODs | 2 rep. |
| Avian influenza - Abs
JGKPT41 |
6 x FD sera (poultry, chicken) | AI01 – AI06 | 2-8oC | Add 350 µL sterile DH2O to each vial if you received a 350 µL volume
Add 700 µL sterile DH2O to each vial if you received a 700 µL volume |
Test immediately upon reconstitution | ELISA
HI |
R.E.F 39-45 | ODs / Titres | 2 rep |
| Inf. bronchitis virus – Abs
JGKPT42 |
6 x Liquid sera (poultry) | IBV01 – IBV06 | 2-8oC
Do not freeze |
Ready to process | Test immediately upon receipt | ELISA
HI |
R.E.F 39-45 | ODs / Titres | 2 rep |
| Newcastle disease – Abs JGKPT43 | 6 x FD sera (poultry) | NDV01 – NDV06 | 2-8oC | Add 350 µL sterile DH2O to each vial | Test immediately upon reconstitution | ELISA
HI |
R.E.F 39-45 | ODs / Titres | 2 rep |
| Salmonella enteritidis – Abs
JGKPT44 |
6 x Liquid poultry sera | SE01 – SE06 | 2-8oC
Do not freeze |
Ready to process | Test immediately upon receipt | ELISA
RPAT |
R.E.F 39-45 | Positive / Negative | 1 rep |
| Mycoplasma gallisepticum - Abs
JGKPT45 |
6 x Liquid sera (poultry) | MG01 – MG06 | 2-8oC
Do not freeze |
Ready to process | Test immediately upon receipt | ELISA
RPAT |
R.E.F 39-45 | Positive / Negative | 1 rep |
| Treatment of samples
No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests None
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 25th of May 2022. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None |
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FD: Freeze-Dried