PT Instructions
22 February 2022 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme Description and Code | Samples | Samples reconstitution | Testing timeframe | Approved test methods | Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| Antibiogram – mastitis pathogens
JGKPT02 |
2 x FD cultures | ATB01M – ATB02M | 2-8oC | Add sterile DH2O:
1 ml to vial 1 350 µL to vial 2 |
Culturing of bacteria should be done Immediately upon reconstitution of the sample | Culturing: micro
Susceptibility: Kirby-Bauer / Disc diffusion (Qualitative) |
R.E.F 02
(supplied) |
Zone Ø in millimeters
(Results: S, I, R) |
Culture: 1 rep
Susceptibility: 3 replicates (supplied discs) |
| 18 x antibiotic discs | 6 x Penicillin 10 IU
6 x Tetracycline 30 µg 6 x Sulphamethoxazole 100 µg |
Commercial discs:
Ready to process |
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| Antimicrobial residues – meat
JGKPT23-25 |
4 x meat pieces | RES-01-F – RES-04-F | 2-8oC | Ready to process | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 23-25
(supplied) |
Presence/Absence of antimicrobials
ID of the antibiotic |
1 rep |
| Antimicrobial residues – milk
JGKPT20-22 |
4 x FD milk (bovine) | RES-01-M – RES-04-M | 2-8oC | Add 2 ml sterile DH2O
to each vial Note: samples and D.H2O must all adjust to room To before use |
Test immediately upon reconstitution | Rapid test
Microbiology Chromatography |
R.E.F 20-22
(supplied) |
Presence/Absence of antimicrobials
ID of the antibiotic |
1 rep |
| Food hygiene – meat (Detection)
JGKPT25-30 |
3 x FD samples per target organism | 1 set each (FH01 – FH03) for:
Salmonella spp. Escherichia coli Escherichia. coli O157 Campylobacter jejuni Listeria monocytogenes |
2-8oC | Add 0.5 ml sterile DH2O
to each vial |
Test immediately upon reconstitution | Microbiology (conventional & PCR) | R.E.F 25-30
(supplied) |
Presence/Absence of target bacteria | 1 rep |
| Mastitis pathogens – milk
JGKPT34 |
2 x FD samples (bovine) | MAST01 – MAST02 | 2-8oC | Add sterile DH2O:
0.5 ml to vial 1 1 ml to vial 2 |
Test immediately upon reconstitution | Culture & ID | R.E.F 33-34
(supplied) |
Target bacteria | 1 rep |
| Milk hygiene: TBC, Coliforms and E. coli counts
JGKPT17-19 |
4 x FD milk | MH01 – MH04 | 2-8oC | Add 10 ml sterile DH2O
to each vial Notes: Samples must adjust to room To for ≥ 30 min before adding D.H2O. D.H2O must be at 37oC in water bath for ≥30 min before use |
Test immediately upon reconstitution | 3M Petri Films
Conventional technique |
R.E.F 17-19 | Counts | 2 rep |
| Treatment of samples
No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests None
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 9th of March 2022. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None |
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FD: Freeze-Dried