PT Instructions
17 September 2018 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme Description and Code | Samples | Samples reconstitution | Testing timeframe | Approved test methods | Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| Avian influenza virus
(Virus isolation) JGKPT47 |
3 x frozen virus suspensions
(1 ml) |
AIVI01 – AIVI03 | Keep frozen
-86oC |
Ready to process | Long term if kept frozen | Virus propagation in EE | R.E.F 47.50
(supplied) |
Presence/Absence of target virus | 1 rep |
| Avian influenza virus
(PCR) JGKPT48 |
3 x frozen virus suspensions
(1 ml) |
AIPCR01 – AIPCR03 | Keep frozen
-86oC |
Ready to process | Long term if kept frozen | PCR | Presence/Absence of target nucleic acid | 1 rep | |
| Avian influenza
(Antibodies) (H5/H6/H7) JGKPT42 And Newcastle disease (Antibodies) JGKPT44 |
6 x FD sera
Note: These samples are for both AI and NDV Test only for conditions you are registered for |
AI/NDV01 – AI/NDV06 | 2-8oC | Add sterile D.H2O
Sample 1: 0.8 ml Sample 2: 1 ml Sample 3: 1 ml Sample 4: 0.8 ml Sample 5: 1 ml Sample 6: 1 ml |
Test immediately after reconstitution | ELISA & HA/HI tests | R.E.F 42-46
(supplied) |
ELISA: ODs & titres
HI: Titres |
ELISA: 2 rep
HI: 2 rep |
| Bovine viral diarrhoea JGKPT40
And Fasciola hepatica (Antibodies) JGKPT41 |
5 x bovine sera
(liquid, 1 ml) |
BVD/FAS01 – BVD/FAS05 | 2-8oC | Ready to process | Test within 5 days of dispatch | ELISA | R.E.F 40-41
(supplied) |
ELISA ODs | 2 rep |
| Infectious bronchitis virus (Antibodies)
JGKPT43 Mycoplasma gallisepticum – chicken (Antibodies) JGKPT46 Salmonella enteritidis (Antibodies) JGKPT45 |
6 x FD sera
Note: These samples are for IBV, MG and SE Test only for conditions you are registered for |
IBV/MG/SE01 – IBV/MG/SE06 | 2-8oC | Add sterile D.H2O
Sample 1: 1 ml Sample 2: 1 ml Sample 3: 1 ml Sample 4: 0.5 ml Sample 5: 1 ml Sample 6: 1 ml |
Test immediately after reconstitution | IBV: ELISA | R.E.F 42-46
(supplied) |
ELISA ODs
RSPA: Positive/Negative |
ELISA: 2 rep RSPA: 1 rep |
| MG: RSPA/ELISA | |||||||||
| SE: RSPA/ELISA | |||||||||
| Newcastle disease virus (Virus isolation)
JGKPT49 |
3 x frozen virus suspensions
(1 ml) |
NDVI01 – NDVI03 | Keep frozen
-86oC |
Ready to process | Long term if kept frozen | Virus propagation in EE | R.E.F 47-50
(supplied) |
Positive/Negative target virus | 1 rep |
| Newcastle disease virus (PCR)
JGKPT50 |
3 x frozen virus suspensions
(1 ml) |
NDPCR01 – NDPCR03 | Keep frozen
-86oC |
Ready to process | Long term if kept frozen | PCR | Positive/Negative target nucleic acid | 1 rep | |
| Salmonella in Poultry
(Agent ID) JGKPT10 |
3 x FD cultures | SAP01 – SAP03 | 2-8oC | Add 0.5 ml sterile D.H2O to each vial | Test immediately after reconstitution | Culturing / microbiological | R.E.F 10-11
(supplied) |
Present/Absent
PLUS ID of the salmonella isolate |
1 rep |
| Salmonella in Mammals
(Agent ID) JGKPT11 |
3 x FD cultures | SAM01 – SAM03 | 2-8oC | Add 0.5 ml sterile D.H2O to each vial | Test immediately after reconstitution | Culturing / microbiological | 1 rep | ||
| Treatment of samples
No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests JGKPT47- JGKPT50 (Virus isolation) must be handled in a biological safety cabinet class 2+
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 15th of October 2018. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
|
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FD: Freeze-Dried