06 August 2018 Run – JGK Lab AfriQA

Scheme reference	Samples			Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
Scheme reference	Type	Identification (as per the labels)	Storage	Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
JGKPT01 Antimicrobial susceptibility (clin. pathogens)	1 x swab 1 x FD culture	ATB01/C: Swine – Nephritis ATB02/C: Sheep – Nervous	2-8^oC	Swab: ready to process FD culture: add 1 ml sterile DH₂O to the vial	Test immediately after reconstitution	Disc diffusion technique	R.E.F 1.2 (supplied)	Zone Ø in millimeters. Report reactions as: Sensitive (“S”), Intermediate (“I”), Resistance (“R”)	Culture ID: 1 repl Susceptibility: JGK discs: 3 repl. Lab discs: 1 repl. (see result sheet)
	Antimicrobial discs (x18)	Enrofloxacin 5 µg (x6) Tetracycline 30 µg (x6) Linco / Spectino 109 µg (x6)	2-8^oC	Discs are ready to use (Commercial discs)	Test immediately after reconstitution	Disc diffusion technique	R.E.F 1.2 (supplied)
JGKPT03 Brucella: RBT	6 FD bovine sera	These samples are for Labs that perform RBT only RBT01 – RBT06	2-8^oC	Add 1 ml sterile DH₂O to the vial	Test within 2 days of reconstitution	RBT only	R.E.F 3.5 (supplied)	Positive/Negative RBT	1 repl.
JGKPT03/04 Brucella: RBT & CFT	6 FD bovine sera	These samples are for Labs that perform both RBT and CFT RBT/CFT01 – RBT/CFT06	2-8^oC	Add 1 ml sterile DH₂O to the vial	Test within 2 days of reconstitution	RBT and CFT (CFT: DAFF approved SOP for SA Labs)	R.E.F 3.5 (supplied)	RBT: Positive/Negative CFT: IU/ml	RBT: 1 repl. CFT: 2 repl.
JGKPT05 Brucella: MRT	4 cow’s milk	CAM01 – CAM04	2-8^oC	NA Ready to process	Preferably immediately but no later than 5 days after dispatch	MRT	R.E.F 3.5 (supplied)	Positive/Negative MRT	1 repl.
JGKPT06 Brucella: Culture & Identification	1 x swab in Amies 2 bovine milk	CAC01: Swab in Amies CAC02 & CAC03: Milk	2-8^oC	NA Ready to process	Preferably immediately but no later than 5 days after dispatch	Conventional microbiology and Molecular technique	R.E.F 06 (supplied)	Presence/Absence: Brucella abortus Strain: Field or vaccine	1 repl.
Slides	3 unstained smears	Slide 1: x 3 Slide 2: x 3 Slide 3: x 3	Room T^o Dust-free	Proceed with staining technique as per Lab SOP	When and as convenient, but before submission deadline	Stamp’s staining technique	R.E.F 06 (supplied)	Positive/Negative for Brucella	1 repl.
JGKPT36-38 Trichomonas and Campylobacter ID by Culture	5 Cultures in transport media	SW01 – SW05	2-8^oC	NA Ready to process	Preferably immediately but no later than 5 days after dispatch	Conventional microbiology	R.E.F 36.9 (supplied)	Presence/Absence: Trichomonas foetus, Campylobacter fetus	1 repl.
JGKPT37 & 39 Trichomonas and Campylobacter ID by PCR	5 Cultures in transport media	SW01 – SW05	2-8^oC	NA Ready to process		Molecular technique	R.E.F 36.9 (supplied)	Presence/Absence: Trichomonas foetus	1 repl.
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory. Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company. Specific environmental conditions required for the participant to conduct tests All cultures must be handled in a biological safety cabinet. Minimum requirement for JGKPT06 (Brucella Culture): class 2+ Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F. Submission of PT results to JGK All results must be submitted on or before the 27^th of August 2018. Any results received after this date will not be included in the analysis. Enquiries For any inquiry, please use the contact details provided above to contact JGK Return of the proficiency test items None