PT Instructions
5 March 2018 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme Description and Code | Samples | Samples reconstitution | Testing timeframe |
Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type |
Identification (as per the labels) |
Storage | |||||||
|
Antibiogram – mastitis pathogens JGKPT02 |
2 x swabs | ATB01M-ATB02M | 2-8oC | Ready to process | Culturing of bacteria should be done Immediately upon receipt |
Culturing: micro Susceptibility: Kirby-Bauer / Disc diffusion |
R.E.F 02 (supplied) |
Zone Ø in millimeters (Results: S, I, R) |
Culture: 1 rep Susceptibility: 3 rep supplied discs; 1 rep own discs |
| 18 x antibiotic discs |
6 x Penicillin 1unit, 6 x Gentamycin 10µg, 6 x Kanamycin 30µg |
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|
Antimicrobial residues – meat JGKPT24-26 |
3 x pieces of meat (25 g) |
RES01F – RES03F | 2-8oC | Ready to process | Test within 5 days of dispatch |
Rapid test Microbiology Chromatography |
R.E.F 24-26 (supplied) |
Presence/Absence of antimicrobials | 1 rep |
|
Antimicrobial residues – milk JGKPT21-23 |
5 x milk (liquid, 4ml) |
RES01M – RES05M | 2-8oC | Ready to process | Test within 5 days of dispatch |
Rapid test Microbiology Chromatography |
R.E.F 21-23 (supplied) |
Presence/Absence of antimicrobials | 1 rep |
|
Avian influenza virus (Virus isolation) JGKPT47 |
3 x frozen virus suspension (1.2ml) |
AIVI01 – AIVI03 | -86oC | Ready to process | Long term if kept frozen | Virus propagation in EE |
R.E.F 47.50 (supplied) |
Presence/Absence of target virus | 1 rep |
|
Avian influenza virus (PCR) JGKPT48 |
3 x frozen virus suspension (1.2ml) |
AIPCR01 – AIPCR03 | -86oC | Ready to process | Long term if kept frozen | Virus propagation in EE |
R.E.F 47.50 (supplied) |
Presence/Absence of target nucleic acid | 1 rep |
|
Avian influenza (Antibodies-(H5/H6/H7) JGKPT42 |
6 x serum (liquid, 450 µl) |
AI01 – AI03 | 2-8oC | Ready to process | Test within 5 days of dispatch | ELISA & HA/HI test |
R.E.F 42-50 (supplied) |
ELISA: ODs HI: Titres |
ELISA: 2 rep HI: 2 rep |
|
Bovine viral diarrhoea and Fasciola hepatica (Antibodies) JGKPT40 and JGKPT41 |
5 x serum (liquid, 0.6 ml) |
BVD&lF01 – BV&LFD05 | 2-8oC | Ready to process | Test within 5 days of dispatch | ELISA |
R.E.F 40-41 (supplied) |
ELISA ODs | 2 rep |
|
Food hygiene – meat (Detection) JGKPT27-31 |
3 x pieces of meat (25 g) |
FH01 – FH03 | 2-8oC | Ready to process | Test within 5 days of dispatch | Microbiology (conventional & automated) |
R.E.F 27-31 (supplied) |
Presence/Absence of target bacteria | 1 rep |
|
Infectious bronchitis virus (Antibodies) JGKPT43 |
6 x serum (liquid, 400 µl) |
IBV01 – IBV06 | 2-8oC | Ready to process | Test within 5 days of dispatch | ELISA |
R.E.F 42-50 (supplied) |
ELISA ODs | 2 rep |
|
Mastitis pathogens – milk JGKPT35 |
2 x cow’s milk (4 ml) |
MAST01 – MAST02 | 2-8oC | Ready to process | Test within 2 days of dispatch | Culture & ID |
R.E.F 34-35 (supplied) |
Target bacteria | 1 rep |
|
Milk hygiene: TBC, Coliforms and E. coli count JGKPT18-20 |
3 FD milk (10 ml) |
TBC01-TBC03 COL01-COL03 EC01-EC03 |
2-8oC |
Add 10 ml sterile DH2O to the vial Mix and test |
Test immediately after reconstitution | 3M Petri Films | R.E.F 18-20 | Counts | 2 rep |
|
Mycoplasma gallisepticum – chicken (Antibodies) JGKPT46 |
6 x serum (liquid, 400 µl) |
MG01 – MG06 | 2-8oC | Ready to process |
Test within 5 days after reconstitution Observe deadline for submission of results |
RSPA/ELISA |
R.E.F 26-31 (supplied) |
RSPA: Positive/Negative ELISA: OD |
RSPA: 1 rep ELISA: 2 rep |
|
Newcastle disease (Antibodies) JGKPT44 |
6 x serum (liquid, 450 µl) |
NDV01 – NDV06 | 2-8oC | Ready to process | Test within 5 days of dispatch | ELISA & HA/HI test |
R.E.F 42-50 (supplied) |
ELISA: ODs HI: Titres |
ELISA: 2 rep HI: 2 rep |
|
Newcastle disease virus (Virus isolation) JGKPT49 |
3 x frozen virus suspensions (1.2 ml) |
NDVI01 – NDVI06 | -86oC | Ready to process | Long term if kept frozen | Virus propagation in EE |
R.E.F 47-50 (supplied) |
Positive/Negative target virus | 1 rep |
|
Newcastle disease virus (PCR) JGKPT50 |
3 x frozen virus suspensions (1.2 ml) |
NDPCR01 – NDPCR03 | -86oC | Ready to process |
Long term if kept frozen Observe deadline for submission of results |
Virus propagation in EE |
R.E.F 76.9 (supplied) |
Positive/Negative target nucleic acid | 1 rep |
|
Salmonella enteritidis (Antibodies) JGKPT45 |
6 x serum (liquid, 400 µl) |
SE01 – SE06 | 2-8oC | Ready to process | Test within 5 days of dispatch | RSPA/ELISA |
R.E.F 42-47 (supplied) |
RSPA: Positive/Negative ELISA: ODs |
RSPA: 1 rep ELISA: 2 rep |
|
Somatic cells counts – milk JGKPT34 |
6 x preserved milk (20 ml) |
SCC01 – SCC06 | 2-8oC | Mix thoroughly before testing | Test within 5 days of dispatch | Somatic cells counter device |
R.E.F 34-35 (supplied) |
Counts | 3 rep |
|
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests JGKPT47- JGKPT50 (Virus isolation) must be handled in a biological safety cabinet class 2+
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 27th of March 2017. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
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FD: Freeze-Dried